Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   H3L97_RS11065 Genome accession   NZ_CP059564
Coordinates   2277555..2278838 (-) Length   427 a.a.
NCBI ID   WP_097114840.1    Uniprot ID   A0A286EG19
Organism   Alysiella filiformis strain DSM 16848     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2272555..2283838
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3L97_RS11055 (H3L97_11055) - 2274986..2276428 (+) 1443 WP_097114842.1 oligopeptide:H+ symporter -
  H3L97_RS11060 (H3L97_11060) pilD 2276637..2277479 (-) 843 WP_097114841.1 A24 family peptidase Machinery gene
  H3L97_RS11065 (H3L97_11065) pilG 2277555..2278838 (-) 1284 WP_097114840.1 type II secretion system F family protein Machinery gene
  H3L97_RS11070 (H3L97_11070) pilF 2278862..2280541 (-) 1680 WP_097114839.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H3L97_RS11075 (H3L97_11075) coaE 2280796..2281437 (+) 642 WP_224446344.1 dephospho-CoA kinase -
  H3L97_RS11080 (H3L97_11080) zapD 2281424..2282179 (+) 756 WP_097114838.1 cell division protein ZapD -
  H3L97_RS11085 (H3L97_11085) - 2282272..2283219 (-) 948 WP_097114837.1 nitronate monooxygenase -

Sequence

Protein


Download         Length: 427 a.a.        Molecular weight: 46535.95 Da        Isoelectric Point: 10.0682

>NTDB_id=470683 H3L97_RS11065 WP_097114840.1 2277555..2278838(-) (pilG) [Alysiella filiformis strain DSM 16848]
MAAATKSKKPVKKAAPKAKGAALKQEPKERGNRYQFEGRNTQTESVVRGEVVAKNEEEARQKLARRSIKVLQITKMKKRR
AKKITAQDITVFTRQLSTMMKAGLPLMQAFDIVAKGHANASMTQLLMNVRNDIEQGTALGDAFARHPKYFDKFYCNLVAA
GEAGGVLDALLDKLATYMEKTQAIKKKVKSALVYPISVVVVAIVLVLVMMMFVLPEFKKVYDGMGAKLPALTQWMMDASD
FFVAQGWIVILGMIGAVVGASFIHKNSPAVQKRVDALLLKLPIFGEIIRKATIARWARTTATLFTAGVPLVEILDSVAGA
AGNIIYEEATHEIRSKVNQGISLTSAMQATDVFPNMVVQMASIGEEAGSLDDMLNKVAEFYEDEVDNAVAMLSSLMEPII
MVVLGSIVGVILIAMYLPLFSMGDALG

Nucleotide


Download         Length: 1284 bp        

>NTDB_id=470683 H3L97_RS11065 WP_097114840.1 2277555..2278838(-) (pilG) [Alysiella filiformis strain DSM 16848]
ATGGCAGCAGCAACAAAGTCTAAAAAGCCAGTTAAAAAAGCCGCACCCAAAGCCAAAGGTGCTGCCTTAAAACAAGAACC
CAAAGAACGCGGCAATCGCTATCAATTTGAAGGGCGCAACACCCAAACCGAATCGGTGGTGCGCGGCGAAGTGGTTGCCA
AAAACGAAGAAGAAGCCCGTCAGAAGCTGGCACGGCGCAGCATTAAGGTGTTGCAAATCACCAAAATGAAAAAACGCCGT
GCCAAGAAAATCACGGCACAAGACATTACCGTGTTCACACGTCAGCTTTCCACCATGATGAAAGCAGGTTTGCCACTTAT
GCAAGCCTTTGACATTGTTGCAAAAGGTCATGCCAACGCCTCTATGACCCAATTGCTGATGAATGTACGCAACGACATTG
AACAGGGTACGGCTTTGGGCGATGCCTTTGCGCGGCACCCCAAATATTTTGACAAATTCTATTGCAACTTGGTGGCAGCA
GGTGAAGCAGGTGGTGTGTTGGACGCGCTTTTGGATAAGCTCGCCACTTATATGGAAAAAACCCAAGCCATTAAAAAGAA
AGTGAAAAGTGCATTGGTTTACCCCATTTCGGTGGTGGTGGTGGCGATTGTGCTGGTGTTGGTGATGATGATGTTCGTGC
TGCCTGAATTTAAAAAGGTGTATGACGGCATGGGCGCGAAACTGCCAGCCTTAACCCAATGGATGATGGACGCATCGGAC
TTTTTTGTGGCACAAGGTTGGATTGTGATTTTGGGTATGATTGGTGCGGTGGTGGGTGCAAGTTTTATCCACAAAAATTC
ACCAGCCGTGCAAAAACGTGTGGACGCGCTGTTGTTGAAATTGCCCATTTTTGGTGAAATCATTCGCAAAGCGACCATTG
CGCGTTGGGCGCGTACCACAGCCACGCTGTTTACAGCAGGTGTGCCTTTGGTGGAAATTTTGGATTCGGTGGCAGGTGCG
GCAGGCAACATCATTTATGAAGAAGCCACGCATGAAATCCGCAGCAAGGTGAATCAGGGTATTTCGCTGACTTCCGCCAT
GCAAGCAACTGATGTTTTCCCTAATATGGTGGTACAAATGGCATCAATTGGTGAAGAAGCAGGTTCTTTGGACGATATGT
TAAACAAAGTTGCCGAATTTTACGAAGATGAGGTGGACAATGCGGTGGCGATGTTGTCATCATTGATGGAACCCATCATC
ATGGTGGTATTGGGCAGTATTGTGGGCGTGATTTTGATTGCCATGTACTTGCCATTGTTCAGCATGGGCGATGCTTTGGG
TTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(296-418)

Type II secretion system (T2SS), protein F (T2SSF)

(92-215)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A286EG19

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

65.816

91.803

0.604
  pilG Neisseria meningitidis 44/76-A

65.306

91.803

0.6
  pilC Legionella pneumophila strain ERS1305867

47.99

93.208

0.447
  pilC Acinetobacter baumannii D1279779

45.052

89.93

0.405
  pilC Pseudomonas stutzeri DSM 10701

44.02

92.037

0.405
  pilC Acinetobacter baylyi ADP1

44.271

89.93

0.398
  pilC Vibrio cholerae strain A1552

39.401

93.911

0.37