Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   H2789_RS01455 Genome accession   NZ_CP059549
Coordinates   307191..307940 (+) Length   249 a.a.
NCBI ID   WP_045433421.1    Uniprot ID   -
Organism   Acinetobacter calcoaceticus strain NCIMB13260     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 302191..312940
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2789_RS01440 (H2789_01445) ponA 302772..305333 (-) 2562 WP_347019401.1 penicillin-binding protein PBP1a -
  H2789_RS01445 (H2789_01450) pilM 305495..306553 (+) 1059 WP_347019402.1 pilus assembly protein PilM Machinery gene
  H2789_RS01450 (H2789_01455) pilN 306553..307194 (+) 642 WP_347019403.1 PilN domain-containing protein Machinery gene
  H2789_RS01455 (H2789_01460) pilO 307191..307940 (+) 750 WP_045433421.1 type 4a pilus biogenesis protein PilO Machinery gene
  H2789_RS01460 (H2789_01465) pilP 307951..308478 (+) 528 WP_045433419.1 pilus assembly protein PilP Machinery gene
  H2789_RS01465 (H2789_01470) pilQ 308541..310694 (+) 2154 WP_347019404.1 type IV pilus secretin PilQ family protein Machinery gene
  H2789_RS01470 (H2789_01475) aroK 310706..311275 (+) 570 WP_005038276.1 shikimate kinase AroK -
  H2789_RS01475 (H2789_01480) aroB 311304..312386 (+) 1083 WP_347019405.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 249 a.a.        Molecular weight: 28090.94 Da        Isoelectric Point: 4.8667

>NTDB_id=470481 H2789_RS01455 WP_045433421.1 307191..307940(+) (pilO) [Acinetobacter calcoaceticus strain NCIMB13260]
MSPDELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFVVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITMEATGDYHAFGAFVSSIAALPRIVTMHDFVVEAAPVKDSKSDIPVLNYSIKAKTYRYMGAAENAEHPENNASASVAPV
SSTANTQSK

Nucleotide


Download         Length: 750 bp        

>NTDB_id=470481 H2789_RS01455 WP_045433421.1 307191..307940(+) (pilO) [Acinetobacter calcoaceticus strain NCIMB13260]
ATGAGTCCGGATGAACTTCATGAGTTGTCGTTAGAGCAAGCTCCATCTAAGAAAAAAAAGATCACTTTAGAAAAATTTCT
TCAGCAATTTAACACACTAGATATGAACAGCTATGGTAGCTGGCCATTATCTGTGAAAATTACTTGCTGGATTTTTATTT
TCTTTGCTGTGTTGGCTTTGGGATATTTTGTTGTTATTCAGCCAAAGCTACAAGCAATTGATAATGCACAGGCACAAGAA
AGTAATTTGTTGAATGAATTTCGAGAAAAAGATTCAAAATTGCGTAACTTGCAGCAATATCAGCTTCAACTTCAAGAGAT
GCAAGCAAACTTTAATCAGCAGTTAGAGCAACTGCCAAAAGAAACCGAAATTCCAAGTTTGGTTGAAGATATTAATTTAA
CTGGGGTGAATTCTGGCCTGAAGTTTAAAAATATCCGTTTAGAAGATGAAGTGAAGCAGGAAATTTTTATTGAACAGCCT
ATTACGATGGAAGCAACCGGCGATTATCACGCTTTTGGTGCTTTTGTCAGTAGTATTGCGGCATTACCACGTATTGTGAC
AATGCATGACTTTGTTGTAGAGGCTGCACCTGTTAAAGATAGTAAGTCAGATATTCCTGTACTTAATTATTCTATTAAAG
CAAAAACTTATCGCTATATGGGCGCCGCAGAAAATGCTGAACATCCGGAAAACAACGCTTCGGCTTCAGTAGCACCTGTT
TCCTCTACTGCTAATACACAGTCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

83.133

100

0.831
  comO Acinetobacter baylyi ADP1

69.758

99.598

0.695