NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	H2N97_RS13145	Genome accession	NZ_CP059497
Coordinates	2681436..2682050 (-)	Length	204 a.a.
NCBI ID	WP_017418180.1	Uniprot ID	-
Organism	Bacillus velezensis strain JSRB 08
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2676436..2687050

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H2N97_RS13115 (H2N97_13115)	rpsT	2676739..2677005 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
H2N97_RS13120 (H2N97_13120)	holA	2677022..2678063 (-)	1042	Protein_2568	DNA polymerase III subunit delta	-
H2N97_RS13125 (H2N97_13125)	-	2678103..2678254 (-)	152	Protein_2569	hypothetical protein	-
H2N97_RS13130 (H2N97_13130)	-	2678295..2678429 (+)	135	WP_003152870.1	YqzM family protein	-
H2N97_RS13135 (H2N97_13135)	comEC	2678448..2680799 (-)	2352	WP_017418179.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
H2N97_RS13140 (H2N97_13140)	-	2680800..2681369 (-)	570	WP_003152868.1	ComE operon protein 2	-
H2N97_RS13145 (H2N97_13145)	comEA	2681436..2682050 (-)	615	WP_017418180.1	helix-hairpin-helix domain-containing protein	Machinery gene
H2N97_RS13150 (H2N97_13150)	comER	2682109..2682930 (+)	822	WP_014305449.1	late competence protein ComER	-
H2N97_RS13155 (H2N97_13155)	-	2682999..2683736 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
H2N97_RS13160 (H2N97_13160)	rsfS	2683733..2684089 (-)	357	WP_003152864.1	ribosome silencing factor	-
H2N97_RS13165 (H2N97_13165)	yqeK	2684086..2684667 (-)	582	WP_017418181.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
H2N97_RS13170 (H2N97_13170)	-	2684657..2685226 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
H2N97_RS13175 (H2N97_13175)	yhbY	2685237..2685527 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
H2N97_RS13180 (H2N97_13180)	aroE	2685521..2686363 (-)	843	WP_017418183.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22218.33 Da Isoelectric Point: 5.4841

                                >NTDB_id=470224 H2N97_RS13145 WP_017418180.1 2681436..2682050(-) (comEA) [Bacillus velezensis strain JSRB 08]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAVGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=470224 H2N97_RS13145 WP_017418180.1 2681436..2682050(-) (comEA) [Bacillus velezensis strain JSRB 08]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG

CAGTGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0470224