Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   H2N74_RS12180 Genome accession   NZ_CP059495
Coordinates   2530177..2531247 (-) Length   356 a.a.
NCBI ID   WP_014418378.1    Uniprot ID   A0AAP4DGV9
Organism   Bacillus velezensis strain JSRB 166     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2525177..2536247
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2N74_RS12130 (H2N74_12130) sipW 2525236..2525820 (-) 585 WP_012117977.1 signal peptidase I SipW -
  H2N74_RS12135 (H2N74_12135) tapA 2525792..2526463 (-) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  H2N74_RS12140 (H2N74_12140) - 2526722..2527051 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  H2N74_RS12145 (H2N74_12145) - 2527092..2527271 (-) 180 WP_003153093.1 YqzE family protein -
  H2N74_RS12150 (H2N74_12150) comGG 2527328..2527705 (-) 378 WP_053284923.1 competence type IV pilus minor pilin ComGG Machinery gene
  H2N74_RS12155 (H2N74_12155) comGF 2527706..2528101 (-) 396 WP_046559876.1 competence type IV pilus minor pilin ComGF -
  H2N74_RS12160 (H2N74_12160) comGE 2528115..2528429 (-) 315 WP_088056452.1 competence type IV pilus minor pilin ComGE -
  H2N74_RS12165 (H2N74_12165) comGD 2528413..2528850 (-) 438 WP_095061019.1 competence type IV pilus minor pilin ComGD Machinery gene
  H2N74_RS12170 (H2N74_12170) comGC 2528840..2529148 (-) 309 WP_014418376.1 competence type IV pilus major pilin ComGC Machinery gene
  H2N74_RS12175 (H2N74_12175) comGB 2529153..2530190 (-) 1038 WP_053284924.1 competence type IV pilus assembly protein ComGB Machinery gene
  H2N74_RS12180 (H2N74_12180) comGA 2530177..2531247 (-) 1071 WP_014418378.1 competence type IV pilus ATPase ComGA Machinery gene
  H2N74_RS12185 (H2N74_12185) - 2531440..2532390 (-) 951 WP_014418379.1 magnesium transporter CorA family protein -
  H2N74_RS12190 (H2N74_12190) - 2532536..2533837 (+) 1302 WP_029973873.1 hemolysin family protein -
  H2N74_RS12195 (H2N74_12195) - 2533875..2534705 (-) 831 WP_017418143.1 STAS domain-containing protein -
  H2N74_RS12205 (H2N74_12205) - 2534912..2535292 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  H2N74_RS12210 (H2N74_12210) - 2535529..2535774 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.81 Da        Isoelectric Point: 9.3485

>NTDB_id=470143 H2N74_RS12180 WP_014418378.1 2530177..2531247(-) (comGA) [Bacillus velezensis strain JSRB 166]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKQQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=470143 H2N74_RS12180 WP_014418378.1 2530177..2531247(-) (comGA) [Bacillus velezensis strain JSRB 166]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCAGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCAATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374