Detailed information NTG0469914

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: JSQ43_RS04000
• Genome accession: NZ_CP069709
• Coordinates: 742588..743247 (-)
• Length: 219 a.a.
• NCBI ID: WP_001221493.1
• Uniprot ID: Q3YXL4
• Organism: Escherichia coli strain EA7
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 737588..748247

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JSQ43_RS03975 (JSQ43_03975)	parE	737679..739571 (+)	1893	WP_000195296.1	DNA topoisomerase IV subunit B	-
JSQ43_RS03980 (JSQ43_03980)	ygiN	739619..739933 (-)	315	WP_000958598.1	putative quinol monooxygenase	-
JSQ43_RS03985 (JSQ43_03985)	mdaB	739964..740545 (-)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
JSQ43_RS03990 (JSQ43_03990)	ygiZ	740864..741196 (+)	333	WP_000917684.1	DUF2645 family protein	-
JSQ43_RS03995 (JSQ43_03995)	qseC	741242..742591 (-)	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
JSQ43_RS04000 (JSQ43_04000)	ciaR	742588..743247 (-)	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	Regulator
JSQ43_RS04005 (JSQ43_04005)	ygiW	743399..743791 (+)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
JSQ43_RS04010 (JSQ43_04010)	ygiV	743844..744326 (+)	483	WP_000183505.1	GyrI-like domain-containing protein	-
JSQ43_RS04015 (JSQ43_04015)	mqsR	744531..744638 (+)	108	Protein_717	type II toxin-antitoxin system toxin MqsR	-
JSQ43_RS04020 (JSQ43_04020)	-	744705..745685 (+)	981	Protein_718	IS5-like element ISKpn26 family transposase	-
JSQ43_RS04025 (JSQ43_04025)	mqsR	745833..746027 (+)	195	Protein_719	type II toxin-antitoxin system toxin MqsR	-
JSQ43_RS04030 (JSQ43_04030)	mqsA	746029..746424 (+)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
JSQ43_RS04035 (JSQ43_04035)	ygiS	746557..748164 (+)	1608	WP_001295629.1	ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24687.63 Da
• Isoelectric Point: 6.9850

>NTDB_id=469914 JSQ43_RS04000 WP_001221493.1 742588..743247(-) (ciaR) [Escherichia coli strain EA7]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=469914 JSQ43_RS04000 WP_001221493.1 742588..743247(-) (ciaR) [Escherichia coli strain EA7]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: Q3YXL4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365