Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   H1Q60_RS02045 Genome accession   NZ_CP059405
Coordinates   384663..385277 (-) Length   204 a.a.
NCBI ID   WP_017418180.1    Uniprot ID   -
Organism   Bacillus velezensis strain MV2     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 379663..390277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1Q60_RS02015 (H1Q60_02015) rpsT 379963..380229 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  H1Q60_RS02020 (H1Q60_02020) holA 380246..381287 (-) 1042 Protein_400 DNA polymerase III subunit delta -
  H1Q60_RS02025 (H1Q60_02025) - 381327..381481 (-) 155 Protein_401 hypothetical protein -
  H1Q60_RS02030 (H1Q60_02030) - 381522..381656 (+) 135 WP_003152870.1 YqzM family protein -
  H1Q60_RS02035 (H1Q60_02035) comEC 381675..384026 (-) 2352 WP_065180883.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  H1Q60_RS02040 (H1Q60_02040) - 384027..384596 (-) 570 WP_003152868.1 ComE operon protein 2 -
  H1Q60_RS02045 (H1Q60_02045) comEA 384663..385277 (-) 615 WP_017418180.1 helix-hairpin-helix domain-containing protein Machinery gene
  H1Q60_RS02050 (H1Q60_02050) comER 385336..386157 (+) 822 WP_014305449.1 late competence protein ComER -
  H1Q60_RS02055 (H1Q60_02055) - 386226..386963 (-) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  H1Q60_RS02060 (H1Q60_02060) rsfS 386960..387316 (-) 357 WP_003152864.1 ribosome silencing factor -
  H1Q60_RS02065 (H1Q60_02065) yqeK 387313..387894 (-) 582 WP_017418181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  H1Q60_RS02070 (H1Q60_02070) - 387884..388453 (-) 570 WP_017418182.1 nicotinate-nucleotide adenylyltransferase -
  H1Q60_RS02075 (H1Q60_02075) yhbY 388464..388754 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  H1Q60_RS02080 (H1Q60_02080) aroE 388748..389590 (-) 843 WP_013352947.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22218.33 Da        Isoelectric Point: 5.4841

>NTDB_id=469412 H1Q60_RS02045 WP_017418180.1 384663..385277(-) (comEA) [Bacillus velezensis strain MV2]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=469412 H1Q60_RS02045 WP_017418180.1 384663..385277(-) (comEA) [Bacillus velezensis strain MV2]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGTGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363