Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   H1D02_RS11675 Genome accession   NZ_CP059328
Coordinates   2348740..2349147 (+) Length   135 a.a.
NCBI ID   WP_006251342.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0728     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2343740..2354147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1D02_RS11650 (H1D02_11700) - 2344242..2345006 (-) 765 WP_006248395.1 TSUP family transporter -
  H1D02_RS11655 (H1D02_11705) proB 2345006..2346097 (-) 1092 WP_240027731.1 glutamate 5-kinase -
  H1D02_RS11660 (H1D02_11710) folA 2346209..2346703 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  H1D02_RS11665 (H1D02_11715) - 2346821..2347054 (+) 234 WP_240027732.1 YgjV family protein -
  H1D02_RS11670 (H1D02_11720) radA 2347109..2348488 (-) 1380 WP_240027733.1 DNA repair protein RadA -
  H1D02_RS11675 (H1D02_11725) pilA 2348740..2349147 (+) 408 WP_006251342.1 pilin Machinery gene
  H1D02_RS11680 (H1D02_11730) pilB 2349168..2350571 (+) 1404 WP_240027734.1 GspE/PulE family protein Machinery gene
  H1D02_RS11685 (H1D02_11735) - 2350555..2351749 (+) 1195 Protein_2263 type II secretion system F family protein -
  H1D02_RS12970 - 2352027..2352233 (+) 207 Protein_2264 peptidase A24 -
  H1D02_RS11690 (H1D02_11745) coaE 2352447..2353082 (+) 636 WP_020831305.1 dephospho-CoA kinase -
  H1D02_RS11695 (H1D02_11750) yacG 2353064..2353249 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 13955.63 Da        Isoelectric Point: 7.5809

>NTDB_id=468709 H1D02_RS11675 WP_006251342.1 2348740..2349147(+) (pilA) [Mannheimia haemolytica strain 0728]
MIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVELCIYNSGGLDNCSGGTNGIQANKTSSNTTKYLNSIAVT
KGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 408 bp        

>NTDB_id=468709 H1D02_RS11675 WP_006251342.1 2348740..2349147(+) (pilA) [Mannheimia haemolytica strain 0728]
ATGATCGTGATTGCGATTATTGCCATTCTTGCCACCATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTT
ATCGGAGTTATTGCAAGCCTCTGCTTCGTATAAATCAGACGTTGAACTCTGTATTTACAACAGTGGCGGGCTGGATAACT
GCTCCGGTGGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACA
AAAGGCGTGATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATAC
GATTAGTTGGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAA
ACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(24-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

64.167

88.889

0.57
  pilA Haemophilus influenzae 86-028NP

51.327

83.704

0.43
  pilA Haemophilus influenzae Rd KW20

50

84.444

0.422