Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   H1D04_RS09775 Genome accession   NZ_CP059326
Coordinates   1923395..1923802 (+) Length   135 a.a.
NCBI ID   WP_006251342.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0745     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1918395..1928802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1D04_RS09750 (H1D04_09670) - 1918899..1919663 (-) 765 WP_275686376.1 TSUP family transporter -
  H1D04_RS09755 (H1D04_09675) proB 1919663..1920754 (-) 1092 WP_240027731.1 glutamate 5-kinase -
  H1D04_RS09760 (H1D04_09680) folA 1920866..1921360 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  H1D04_RS09765 (H1D04_09685) - 1921479..1921709 (+) 231 Protein_1870 YgjV family protein -
  H1D04_RS09770 (H1D04_09690) radA 1921764..1923143 (-) 1380 WP_006251344.1 DNA repair protein RadA -
  H1D04_RS09775 (H1D04_09695) pilA 1923395..1923802 (+) 408 WP_006251342.1 pilin Machinery gene
  H1D04_RS09780 (H1D04_09700) pilB 1923823..1925226 (+) 1404 WP_240027734.1 GspE/PulE family protein Machinery gene
  H1D04_RS09785 (H1D04_09705) - 1925210..1926403 (+) 1194 Protein_1874 type II secretion system F family protein -
  H1D04_RS13010 - 1926681..1926887 (+) 207 Protein_1875 peptidase A24 -
  H1D04_RS09790 (H1D04_09715) coaE 1927098..1927733 (+) 636 WP_275686919.1 dephospho-CoA kinase -
  H1D04_RS09795 (H1D04_09720) yacG 1927715..1927900 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 13955.63 Da        Isoelectric Point: 7.5809

>NTDB_id=468662 H1D04_RS09775 WP_006251342.1 1923395..1923802(+) (pilA) [Mannheimia haemolytica strain 0745]
MIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVELCIYNSGGLDNCSGGTNGIQANKTSSNTTKYLNSIAVT
KGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 408 bp        

>NTDB_id=468662 H1D04_RS09775 WP_006251342.1 1923395..1923802(+) (pilA) [Mannheimia haemolytica strain 0745]
ATGATCGTGATTGCGATTATTGCCATTCTTGCCACCATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTT
ATCGGAGTTATTGCAAGCCTCTGCTTCGTATAAATCAGACGTTGAACTCTGTATTTACAACAGTGGCGGGCTGGATAACT
GCTCCGGTGGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACA
AAAGGCGTGATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATAC
GATTAGTTGGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAA
ACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(24-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

64.167

88.889

0.57
  pilA Haemophilus influenzae 86-028NP

51.327

83.704

0.43
  pilA Haemophilus influenzae Rd KW20

50

84.444

0.422