Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   H1D05_RS08990 Genome accession   NZ_CP059325
Coordinates   1813839..1814246 (+) Length   135 a.a.
NCBI ID   WP_240020848.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0746     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1808839..1819246
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1D05_RS08965 (H1D05_08960) - 1809351..1810115 (-) 765 WP_240019410.1 TSUP family transporter -
  H1D05_RS08970 (H1D05_08965) proB 1810115..1811200 (-) 1086 WP_240019412.1 glutamate 5-kinase -
  H1D05_RS08975 (H1D05_08970) folA 1811312..1811806 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  H1D05_RS08980 (H1D05_08975) - 1811925..1812155 (+) 231 Protein_1730 YgjV family protein -
  H1D05_RS08985 (H1D05_08980) radA 1812210..1813589 (-) 1380 WP_240019414.1 DNA repair protein RadA -
  H1D05_RS08990 (H1D05_08985) pilA 1813839..1814246 (+) 408 WP_240020848.1 pilin Machinery gene
  H1D05_RS08995 (H1D05_08990) - 1814267..1814710 (+) 444 Protein_1733 pilus assembly protein PilB -
  H1D05_RS09000 (H1D05_08995) pilC 1814716..1815912 (+) 1197 WP_240019415.1 type II secretion system F family protein Machinery gene
  H1D05_RS09005 - 1815915..1816118 (+) 204 WP_240019416.1 hypothetical protein -
  H1D05_RS14255 - 1816190..1816396 (+) 207 Protein_1736 peptidase A24 -
  H1D05_RS09010 (H1D05_09005) coaE 1816610..1817245 (+) 636 WP_006248405.1 dephospho-CoA kinase -
  H1D05_RS09015 (H1D05_09010) yacG 1817227..1817412 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  H1D05_RS09020 (H1D05_09015) holA 1817413..1818438 (-) 1026 WP_240020849.1 DNA polymerase III subunit delta -
  H1D05_RS09025 (H1D05_09020) lptE 1818481..1818969 (-) 489 WP_006248408.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 13941.60 Da        Isoelectric Point: 7.5809

>NTDB_id=468637 H1D05_RS08990 WP_240020848.1 1813839..1814246(+) (pilA) [Mannheimia haemolytica strain 0746]
MIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSGGTNGIQANKTSSNTTKYLNSIAVT
KGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 408 bp        

>NTDB_id=468637 H1D05_RS08990 WP_240020848.1 1813839..1814246(+) (pilA) [Mannheimia haemolytica strain 0746]
ATGATCGTGATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTT
ATCGGAGTTATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACT
GCTCCGGTGGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACA
AAAGGCGTGATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATAC
GATTAGTTGGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAA
ACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(24-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

64.167

88.889

0.57
  pilA Haemophilus influenzae 86-028NP

51.304

85.185

0.437
  pilA Haemophilus influenzae Rd KW20

50

85.926

0.43