Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HS056_RS10635 Genome accession   NZ_CP059242
Coordinates   2087997..2088476 (+) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 0005     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2082997..2093476
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS056_RS10610 (HS056_10525) - 2083570..2084340 (-) 771 WP_409040713.1 TSUP family transporter -
  HS056_RS10615 (HS056_10530) proB 2084340..2085425 (-) 1086 WP_409040714.1 glutamate 5-kinase -
  HS056_RS10620 (HS056_10535) folA 2085537..2086031 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  HS056_RS10625 (HS056_10540) - 2086150..2086380 (+) 231 WP_006248398.1 YgjV family protein -
  HS056_RS10630 (HS056_10545) radA 2086435..2087817 (-) 1383 WP_409036533.1 DNA repair protein RadA -
  HS056_RS10635 (HS056_10550) pilA 2087997..2088476 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  HS056_RS10640 (HS056_10555) - 2088497..2088940 (+) 444 Protein_2050 pilus assembly protein PilB -
  HS056_RS10645 (HS056_10560) pilC 2088946..2090142 (+) 1197 WP_240019415.1 type II secretion system F family protein Machinery gene
  HS056_RS10650 - 2090145..2090348 (+) 204 WP_240019416.1 hypothetical protein -
  HS056_RS10655 - 2090419..2090625 (+) 207 Protein_2053 peptidase A24 -
  HS056_RS10660 (HS056_10570) coaE 2090839..2091474 (+) 636 WP_409038838.1 dephospho-CoA kinase -
  HS056_RS10665 (HS056_10575) yacG 2091456..2091641 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  HS056_RS10670 (HS056_10580) holA 2091642..2092667 (-) 1026 WP_409038164.1 DNA polymerase III subunit delta -
  HS056_RS10675 (HS056_10585) lptE 2092710..2093228 (-) 519 WP_409037689.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=468083 HS056_RS10635 WP_006248401.1 2087997..2088476(+) (pilA) [Mannheimia haemolytica strain 0005]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=468083 HS056_RS10635 WP_006248401.1 2087997..2088476(+) (pilA) [Mannheimia haemolytica strain 0005]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491