Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HS062_RS10920 Genome accession   NZ_CP059236
Coordinates   2153309..2153716 (+) Length   135 a.a.
NCBI ID   WP_006251342.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0593     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2148309..2158716
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS062_RS10895 (HS062_10870) - 2148813..2149577 (-) 765 WP_006248395.1 TSUP family transporter -
  HS062_RS10900 (HS062_10875) proB 2149577..2150668 (-) 1092 WP_240027731.1 glutamate 5-kinase -
  HS062_RS10905 (HS062_10880) folA 2150780..2151274 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  HS062_RS10910 (HS062_10885) - 2151393..2151623 (+) 231 Protein_2103 YgjV family protein -
  HS062_RS10915 (HS062_10890) radA 2151678..2153057 (-) 1380 WP_240027733.1 DNA repair protein RadA -
  HS062_RS10920 (HS062_10895) pilA 2153309..2153716 (+) 408 WP_006251342.1 pilin Machinery gene
  HS062_RS10925 (HS062_10900) pilB 2153737..2155140 (+) 1404 WP_240027734.1 GspE/PulE family protein Machinery gene
  HS062_RS10930 (HS062_10905) - 2155124..2156318 (+) 1195 Protein_2107 type II secretion system F family protein -
  HS062_RS10935 - 2156597..2156803 (+) 207 Protein_2108 peptidase A24 -
  HS062_RS10940 (HS062_10915) coaE 2157017..2157652 (+) 636 WP_275686919.1 dephospho-CoA kinase -
  HS062_RS10945 (HS062_10920) yacG 2157634..2157819 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 13955.63 Da        Isoelectric Point: 7.5809

>NTDB_id=467950 HS062_RS10920 WP_006251342.1 2153309..2153716(+) (pilA) [Mannheimia haemolytica strain 0593]
MIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVELCIYNSGGLDNCSGGTNGIQANKTSSNTTKYLNSIAVT
KGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 408 bp        

>NTDB_id=467950 HS062_RS10920 WP_006251342.1 2153309..2153716(+) (pilA) [Mannheimia haemolytica strain 0593]
ATGATCGTGATTGCGATTATTGCCATTCTTGCCACCATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTT
ATCGGAGTTATTGCAAGCCTCTGCTTCGTATAAATCAGACGTTGAACTCTGTATTTACAACAGTGGCGGGCTGGATAACT
GCTCCGGTGGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACA
AAAGGCGTGATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATAC
GATTAGTTGGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAA
ACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(24-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

64.167

88.889

0.57
  pilA Haemophilus influenzae 86-028NP

51.327

83.704

0.43
  pilA Haemophilus influenzae Rd KW20

50

84.444

0.422