Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   I6J78_RS05545 Genome accession   NZ_CP069542
Coordinates   1183212..1183871 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain FDAARGOS_1248     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1178212..1188871
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J78_RS05520 (I6J78_05525) - 1178954..1179901 (+) 948 WP_001305988.1 iron-siderophore ABC transporter substrate-binding protein -
  I6J78_RS05525 (I6J78_05530) - 1179898..1180785 (+) 888 WP_000614957.1 MurR/RpiR family transcriptional regulator -
  I6J78_RS05530 (I6J78_05535) ygiN 1180830..1181144 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  I6J78_RS05535 (I6J78_05540) mdaB 1181175..1181756 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  I6J78_RS05540 (I6J78_05545) qseC 1181866..1183215 (-) 1350 WP_000673373.1 quorum sensing histidine kinase QseC -
  I6J78_RS05545 (I6J78_05550) ciaR 1183212..1183871 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  I6J78_RS05550 (I6J78_05555) ygiW 1184023..1184415 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  I6J78_RS05555 (I6J78_05560) ygiV 1184468..1184950 (+) 483 WP_000183504.1 GyrI-like domain-containing protein -
  I6J78_RS05560 (I6J78_05565) parC 1185496..1187754 (+) 2259 WP_204148156.1 DNA topoisomerase IV subunit A -
  I6J78_RS05565 (I6J78_05570) plsC 1187988..1188725 (+) 738 WP_000965722.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=467949 I6J78_RS05545 WP_001221493.1 1183212..1183871(-) (ciaR) [Escherichia coli strain FDAARGOS_1248]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=467949 I6J78_RS05545 WP_001221493.1 1183212..1183871(-) (ciaR) [Escherichia coli strain FDAARGOS_1248]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGTGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGTTAGCGGAACGTGTAGAGGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAACTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACTCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365