Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HS063_RS09570 Genome accession   NZ_CP059235
Coordinates   1886843..1887325 (+) Length   160 a.a.
NCBI ID   WP_275672167.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0596     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1881843..1892325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS063_RS09545 (HS063_09460) - 1882419..1883183 (-) 765 WP_409038955.1 TSUP family transporter -
  HS063_RS09550 (HS063_09465) proB 1883183..1884268 (-) 1086 WP_240019412.1 glutamate 5-kinase -
  HS063_RS09555 (HS063_09470) folA 1884380..1884874 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  HS063_RS09560 (HS063_09475) - 1884993..1885226 (+) 234 WP_409038956.1 YgjV family protein -
  HS063_RS09565 (HS063_09480) radA 1885281..1886663 (-) 1383 WP_409038957.1 DNA repair protein RadA -
  HS063_RS09570 (HS063_09485) pilA 1886843..1887325 (+) 483 WP_275672167.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HS063_RS09575 (HS063_09490) - 1887346..1887789 (+) 444 Protein_1839 pilus assembly protein PilB -
  HS063_RS09580 (HS063_09495) pilC 1887795..1888991 (+) 1197 WP_409037688.1 type II secretion system F family protein Machinery gene
  HS063_RS09585 - 1889269..1889475 (+) 207 Protein_1841 peptidase A24 -
  HS063_RS09590 (HS063_09505) coaE 1889689..1890324 (+) 636 WP_006248405.1 dephospho-CoA kinase -
  HS063_RS09595 (HS063_09510) yacG 1890306..1890491 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  HS063_RS09600 (HS063_09515) holA 1890492..1891517 (-) 1026 WP_409039408.1 DNA polymerase III subunit delta -
  HS063_RS09605 (HS063_09520) lptE 1891560..1892048 (-) 489 WP_006248408.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 160 a.a.        Molecular weight: 16960.03 Da        Isoelectric Point: 8.7964

>NTDB_id=467925 HS063_RS09570 WP_275672167.1 1886843..1887325(+) (pilA) [Mannheimia haemolytica strain 0596]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQAXSASYKSDVEVCIYNSGGLDNCS
GGTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 483 bp        

>NTDB_id=467925 HS063_RS09570 WP_275672167.1 1886843..1887325(+) (pilA) [Mannheimia haemolytica strain 0596]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCTNNATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCC
GGTGGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGG
CGTGATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTA
GTTGGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGC
TAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

65.625

100

0.656
  pilA Haemophilus influenzae 86-028NP

56.028

88.125

0.494
  pilA Haemophilus influenzae Rd KW20

54.545

89.375

0.487