Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HS071_RS05595 Genome accession   NZ_CP059228
Coordinates   1115159..1115638 (+) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 0715     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1110159..1120638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS071_RS05570 (HS071_05560) - 1110742..1111506 (-) 765 WP_409037685.1 TSUP family transporter -
  HS071_RS05575 (HS071_05565) proB 1111506..1112591 (-) 1086 WP_409037686.1 glutamate 5-kinase -
  HS071_RS05580 (HS071_05570) folA 1112703..1113197 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  HS071_RS05585 (HS071_05575) - 1113315..1113545 (+) 231 WP_006248398.1 YgjV family protein -
  HS071_RS05590 (HS071_05580) radA 1113600..1114979 (-) 1380 WP_240019414.1 DNA repair protein RadA -
  HS071_RS05595 (HS071_05585) pilA 1115159..1115638 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  HS071_RS05600 (HS071_05590) - 1115659..1116102 (+) 444 Protein_1089 pilus assembly protein PilB -
  HS071_RS05605 (HS071_05595) pilC 1116108..1117304 (+) 1197 WP_409037688.1 type II secretion system F family protein Machinery gene
  HS071_RS05610 - 1117582..1117788 (+) 207 Protein_1091 peptidase A24 -
  HS071_RS05615 (HS071_05605) coaE 1118002..1118637 (+) 636 WP_006248405.1 dephospho-CoA kinase -
  HS071_RS05620 (HS071_05610) yacG 1118619..1118804 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  HS071_RS05625 (HS071_05615) holA 1118805..1119830 (-) 1026 WP_409042602.1 DNA polymerase III subunit delta -
  HS071_RS05630 (HS071_05620) lptE 1119873..1120361 (-) 489 WP_409042553.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=467826 HS071_RS05595 WP_006248401.1 1115159..1115638(+) (pilA) [Mannheimia haemolytica strain 0715]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=467826 HS071_RS05595 WP_006248401.1 1115159..1115638(+) (pilA) [Mannheimia haemolytica strain 0715]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491