Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   HZ322_RS04840 Genome accession   NZ_CP059049
Coordinates   984885..986207 (-) Length   440 a.a.
NCBI ID   WP_017864427.1    Uniprot ID   A0AAE4SZ64
Organism   Lactococcus lactis strain N8     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 979885..991207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZ322_RS04810 (HZ322_04810) - 981028..981996 (+) 969 WP_003130649.1 PhoH family protein -
  HZ322_RS04815 (HZ322_04815) - 982054..982533 (+) 480 WP_003130650.1 NUDIX hydrolase -
  HZ322_RS04820 (HZ322_04820) ybeY 982685..983173 (+) 489 WP_003130652.1 rRNA maturation RNase YbeY -
  HZ322_RS04825 (HZ322_04825) - 983157..983612 (+) 456 WP_003130653.1 diacylglycerol kinase family protein -
  HZ322_RS04830 (HZ322_04830) - 983626..984204 (+) 579 WP_017864426.1 nucleotidyltransferase family protein -
  HZ322_RS12685 comFC 984238..984642 (-) 405 WP_226898158.1 ComF family protein Machinery gene
  HZ322_RS04840 (HZ322_04840) comFA 984885..986207 (-) 1323 WP_017864427.1 DEAD/DEAH box helicase Machinery gene
  HZ322_RS04845 (HZ322_04845) - 986264..986893 (+) 630 WP_003130660.1 YigZ family protein -
  HZ322_RS04850 (HZ322_04850) - 987027..987524 (+) 498 WP_012897708.1 VanZ family protein -
  HZ322_RS04855 (HZ322_04855) - 987572..988591 (+) 1020 WP_012897709.1 DUF475 domain-containing protein -
  HZ322_RS04860 (HZ322_04860) - 988701..989453 (+) 753 WP_012897710.1 bifunctional metallophosphatase/5'-nucleotidase -
  HZ322_RS04865 (HZ322_04865) glyQ 989739..990692 (+) 954 WP_003130667.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50215.74 Da        Isoelectric Point: 10.1065

>NTDB_id=466804 HZ322_RS04840 WP_017864427.1 984885..986207(-) (comFA) [Lactococcus lactis strain N8]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVLYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDNYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKITSVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=466804 HZ322_RS04840 WP_017864427.1 984885..986207(-) (comFA) [Lactococcus lactis strain N8]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAGCGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTACTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAACTATTTCCGAGCTCCATTAATAATCATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTACTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(114-233)

Helicase conserved C-terminal domain (Helicase_C)

(300-403)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74

90.909

0.673
  comFA/cflA Streptococcus mitis SK321

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae Rx1

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae D39

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae R6

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae TIGR4

52.01

90.455

0.47
  comFA/cflA Streptococcus mitis NCTC 12261

51.256

90.455

0.464
  comFA Bacillus subtilis subsp. subtilis str. 168

37.029

100

0.38