Detailed information NTG0466756

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: H0A38_RS05135
• Genome accession: NZ_CP059048
• Coordinates: 1039616..1040938 (-)
• Length: 440 a.a.
• NCBI ID: WP_129881589.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain LAC460
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1034616..1045938

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H0A38_RS05105 (H0A38_05075)	-	1035759..1036727 (+)	969	WP_003130649.1	PhoH family protein	-
H0A38_RS05110 (H0A38_05080)	-	1036785..1037264 (+)	480	WP_003130650.1	NUDIX hydrolase	-
H0A38_RS05115 (H0A38_05085)	ybeY	1037416..1037904 (+)	489	WP_003130652.1	rRNA maturation RNase YbeY	-
H0A38_RS05120 (H0A38_05090)	-	1037888..1038343 (+)	456	WP_129881588.1	diacylglycerol kinase family protein	-
H0A38_RS05125 (H0A38_05095)	-	1038357..1038935 (+)	579	WP_021722515.1	nucleotidyltransferase family protein	-
H0A38_RS05130	comFC	1038969..1039373 (-)	405	WP_021722514.1	ComF family protein	Machinery gene
H0A38_RS05135 (H0A38_05105)	comFA	1039616..1040938 (-)	1323	WP_129881589.1	DEAD/DEAH box helicase	Machinery gene
H0A38_RS05140 (H0A38_05110)	-	1040995..1041624 (+)	630	WP_129881590.1	YigZ family protein	-
H0A38_RS05145 (H0A38_05115)	-	1041758..1042255 (+)	498	WP_058208413.1	VanZ family protein	-
H0A38_RS05150 (H0A38_05120)	-	1042303..1043322 (+)	1020	WP_226319407.1	DUF475 domain-containing protein	-
H0A38_RS05155 (H0A38_05125)	-	1043432..1044184 (+)	753	WP_129881592.1	bifunctional metallophosphatase/5'-nucleotidase	-
H0A38_RS05160 (H0A38_05130)	glyQ	1044469..1045422 (+)	954	WP_003130667.1	glycine--tRNA ligase subunit alpha	-

Sequence

Protein

• Length: 440 a.a.
• Molecular weight: 50188.74 Da
• Isoelectric Point: 10.1468

>NTDB_id=466756 H0A38_RS05135 WP_129881589.1 1039616..1040938(-) (comFA) [Lactococcus lactis strain LAC460]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNPILGITSISSNKIKCNRCGTVHIKKSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide

• Length: 1323 bp

>NTDB_id=466756 H0A38_RS05135 WP_129881589.1 1039616..1040938(-) (comFA) [Lactococcus lactis strain LAC460]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAGCGAAGGTGAATCCAATTTTGGGAATAACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAAATCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGGTTTTCATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (114-232)

• Helicase conserved C-terminal domain (Helicase_C) (300-403)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	74.5	90.909	0.677
comFA/cflA	Streptococcus mitis SK321	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae Rx1	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae D39	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae R6	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae TIGR4	52.01	90.455	0.47
comFA/cflA	Streptococcus mitis NCTC 12261	51.256	90.455	0.464
comFA	Bacillus subtilis subsp. subtilis str. 168	37.251	100	0.382