Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   MS7_RS08315 Genome accession   NC_017351
Coordinates   1652721..1653218 (-) Length   165 a.a.
NCBI ID   WP_029263717.1    Uniprot ID   -
Organism   Staphylococcus aureus subsp. aureus 11819-97     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1647721..1658218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MS7_RS08290 (MS7_1552) gcvPB 1647883..1649355 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  MS7_RS08295 (MS7_1553) gcvPA 1649348..1650694 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  MS7_RS08300 (MS7_1554) gcvT 1650714..1651805 (-) 1092 WP_000093353.1 glycine cleavage system aminomethyltransferase GcvT -
  MS7_RS08305 (MS7_1555) - 1651964..1652488 (-) 525 WP_001015123.1 shikimate kinase -
  MS7_RS08310 - 1652478..1652624 (-) 147 WP_001789879.1 hypothetical protein -
  MS7_RS08315 (MS7_1556) comGF 1652721..1653218 (-) 498 WP_029263717.1 competence type IV pilus minor pilin ComGF Machinery gene
  MS7_RS08320 (MS7_1557) comGE 1653136..1653435 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  MS7_RS08325 (MS7_1558) comGD 1653422..1653868 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  MS7_RS08330 (MS7_1559) comGC 1653846..1654157 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  MS7_RS08335 (MS7_1560) comGB 1654171..1655241 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  MS7_RS08340 (MS7_1561) comGA 1655213..1656187 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  MS7_RS08345 (MS7_1562) - 1656239..1656862 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  MS7_RS08350 (MS7_1563) - 1656859..1657188 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  MS7_RS08355 (MS7_1564) - 1657188..1658174 (-) 987 WP_000161312.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19224.98 Da        Isoelectric Point: 10.2192

>NTDB_id=46643 MS7_RS08315 WP_029263717.1 1652721..1653218(-) (comGF) [Staphylococcus aureus subsp. aureus 11819-97]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTNVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTVNIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=46643 MS7_RS08315 WP_029263717.1 1652721..1653218(-) (comGF) [Staphylococcus aureus subsp. aureus 11819-97]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAAATGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGTAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(70-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

96.97

100

0.97
  comGF Staphylococcus aureus MW2

96.97

100

0.97

Multiple sequence alignment