Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   HYO64_RS18900 Genome accession   NZ_CP058729
Coordinates   3538751..3539611 (+) Length   286 a.a.
NCBI ID   WP_001152277.1    Uniprot ID   A0A009HWK1
Organism   Acinetobacter baumannii strain M164-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3533751..3544611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HYO64_RS16905 secG 3534356..3534685 (-) 330 WP_000555570.1 preprotein translocase subunit SecG -
  HYO64_RS16910 tpiA 3534698..3535492 (-) 795 WP_114155284.1 triose-phosphate isomerase -
  HYO64_RS16915 pilB 3535783..3537495 (+) 1713 WP_114155282.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HYO64_RS16920 pilC 3537525..3538751 (+) 1227 WP_000279216.1 type II secretion system F family protein Machinery gene
  HYO64_RS18900 pilD 3538751..3539611 (+) 861 WP_001152277.1 prepilin peptidase Machinery gene
  HYO64_RS16935 coaE 3539613..3540209 (+) 597 WP_000874125.1 dephospho-CoA kinase -
  HYO64_RS16940 - 3540206..3541120 (-) 915 WP_000075015.1 DMT family transporter -
  HYO64_RS16945 rlmB 3541156..3541905 (-) 750 WP_001118149.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  HYO64_RS16950 - 3542007..3542333 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -
  HYO64_RS16955 - 3542410..3543720 (-) 1311 WP_114155280.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32038.43 Da        Isoelectric Point: 7.1041

>NTDB_id=465091 HYO64_RS18900 WP_001152277.1 3538751..3539611(+) (pilD) [Acinetobacter baumannii strain M164-3]
MQDIIAYFIQNLTALYIAVALVSLCIGSFLNVVIYRTPKMMEQDWQQECQMLLNPEQPIIDHEKLTLSKPASSCPACQQP
IRWYQNIPVISWLVLRGKCGHCQHPISIRYPAIELLTMLCSLVVVIVFGPTIQMLFGLVLTWVLIALTFIDFDTQLLPDR
FTLPLAALGLGINTFNIYTSPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
LGAIIGIILLKLRNDNQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG

Nucleotide


Download         Length: 861 bp        

>NTDB_id=465091 HYO64_RS18900 WP_001152277.1 3538751..3539611(+) (pilD) [Acinetobacter baumannii strain M164-3]
ATGCAAGACATCATTGCGTATTTTATTCAAAACTTAACTGCACTTTATATTGCTGTTGCACTCGTGAGCCTATGTATCGG
TAGCTTTCTTAATGTAGTGATTTACCGCACGCCAAAAATGATGGAGCAAGATTGGCAGCAAGAATGCCAAATGCTGCTCA
ATCCTGAGCAACCCATTATTGATCATGAGAAGTTAACTTTAAGTAAGCCTGCTTCATCGTGCCCTGCATGTCAGCAACCG
ATCCGTTGGTATCAAAATATTCCTGTTATAAGCTGGCTTGTGTTAAGAGGAAAATGTGGTCATTGCCAGCACCCGATTAG
TATTCGCTATCCAGCCATCGAACTACTCACTATGTTATGTTCATTAGTAGTCGTCATAGTGTTTGGTCCAACCATACAAA
TGCTTTTTGGACTCGTCCTCACATGGGTACTGATAGCCCTTACCTTTATTGATTTCGATACACAATTATTGCCTGATCGC
TTTACCTTACCTTTAGCTGCACTCGGCTTAGGTATTAATACCTTTAATATTTATACCTCACCCAACTCAGCCATTTGGGG
TTATCTCATTGGTTTCCTATGTCTTTGGATTGTCTATTACTTATTTAAAGTGATCACTGGCAAAGAAGGTATGGGCTACG
GCGACTTTAAATTGCTTGCAGCATTAGGTGCATGGATGGGGCCATTGATGCTGCCGTTAATTGTGTTATTGTCATCGTTA
CTTGGCGCAATCATTGGCATCATTTTATTAAAATTAAGAAATGACAATCAGCCTTTTGCTTTTGGGCCATATATTGCCAT
TGCTGGTTGGGTTGCCTTTTTATGGGGTGATCAGATTATGAAAATTTATTTGGGAGGTTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(22-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009HWK1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

98.951

100

0.99
  pilD Acinetobacter nosocomialis M2

98.601

100

0.986
  pilD Vibrio cholerae strain A1552

54.044

95.105

0.514
  pilD Vibrio campbellii strain DS40M4

52.768

94.755

0.5
  pilD Neisseria gonorrhoeae MS11

47.727

92.308

0.441