Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   HW113_RS06660 Genome accession   NZ_CP058312
Coordinates   1306625..1307599 (+) Length   324 a.a.
NCBI ID   WP_000697228.1    Uniprot ID   A0A0D3Q868
Organism   Staphylococcus aureus strain BLR-DV     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1301625..1312599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW113_RS06625 (HW113_06625) rpmG 1302088..1302237 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  HW113_RS06630 (HW113_06630) - 1302446..1302985 (+) 540 WP_000164545.1 5-formyltetrahydrofolate cyclo-ligase -
  HW113_RS06635 (HW113_06635) - 1302997..1304457 (+) 1461 WP_112366461.1 rhomboid family intramembrane serine protease -
  HW113_RS06640 (HW113_06640) - 1304438..1304641 (+) 204 WP_000087561.1 YqgQ family protein -
  HW113_RS06645 (HW113_06645) - 1304638..1305624 (+) 987 WP_000161314.1 ROK family glucokinase -
  HW113_RS06650 (HW113_06650) - 1305624..1305953 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  HW113_RS06655 (HW113_06655) - 1305950..1306573 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  HW113_RS06660 (HW113_06660) comGA 1306625..1307599 (+) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  HW113_RS06665 (HW113_06665) comGB 1307571..1308641 (+) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  HW113_RS06670 (HW113_06670) comGC 1308655..1308966 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  HW113_RS06675 (HW113_06675) comGD 1308944..1309390 (+) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  HW113_RS06680 (HW113_06680) comGE 1309377..1309676 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  HW113_RS06685 (HW113_06685) comGF 1309594..1310091 (+) 498 WP_001803317.1 competence type IV pilus minor pilin ComGF Machinery gene
  HW113_RS06690 (HW113_06690) - 1310188..1310334 (+) 147 WP_001789879.1 hypothetical protein -
  HW113_RS06695 (HW113_06695) - 1310324..1310848 (+) 525 WP_001015117.1 shikimate kinase -
  HW113_RS06700 (HW113_06700) gcvT 1311007..1312098 (+) 1092 WP_000093351.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36865.01 Da        Isoelectric Point: 9.0399

>NTDB_id=463695 HW113_RS06660 WP_000697228.1 1306625..1307599(+) (comGA) [Staphylococcus aureus strain BLR-DV]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
HKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=463695 HW113_RS06660 WP_000697228.1 1306625..1307599(+) (comGA) [Staphylococcus aureus strain BLR-DV]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCTTTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACCACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATAAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
CATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D3Q868

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.074

100

0.991
  comGA Staphylococcus aureus N315

99.074

100

0.991