Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   JNN58_RS09960 Genome accession   NZ_CP068827
Coordinates   2091235..2091894 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain RIVM_C030371     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2086235..2096894
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNN58_RS09945 (JNN58_09945) ygiS 2088440..2090047 (-) 1608 WP_001551657.1 ABC transporter substrate-binding protein -
  JNN58_RS09950 (JNN58_09950) ygiV 2090156..2090638 (-) 483 WP_000183492.1 GyrI-like domain-containing protein -
  JNN58_RS09955 (JNN58_09955) ygiW 2090691..2091083 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  JNN58_RS09960 (JNN58_09960) ciaR 2091235..2091894 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  JNN58_RS09965 (JNN58_09965) qseC 2091891..2093240 (+) 1350 WP_001551658.1 quorum sensing histidine kinase QseC -
  JNN58_RS09970 (JNN58_09970) ygiZ 2093289..2093618 (-) 330 WP_001551659.1 DUF2645 family protein -
  JNN58_RS09975 (JNN58_09975) mdaB 2093976..2094557 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  JNN58_RS09980 (JNN58_09980) ygiN 2094588..2094902 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  JNN58_RS09985 (JNN58_09985) - 2094947..2095834 (-) 888 WP_000614953.1 MurR/RpiR family transcriptional regulator -
  JNN58_RS09990 (JNN58_09990) - 2095831..2096778 (-) 948 WP_001305988.1 iron-siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=463631 JNN58_RS09960 WP_001221493.1 2091235..2091894(+) (ciaR) [Escherichia coli strain RIVM_C030371]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=463631 JNN58_RS09960 WP_001221493.1 2091235..2091894(+) (ciaR) [Escherichia coli strain RIVM_C030371]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGTGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGTTAGCGGAACGTGTAGAGGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAACTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACTCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365