Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGE/cglE   Type   Machinery gene
Locus tag   HW271_RS07625 Genome accession   NZ_CP058258
Coordinates   1556997..1557299 (-) Length   100 a.a.
NCBI ID   WP_178895505.1    Uniprot ID   A0A7H8ZBB4
Organism   Streptococcus sp. oral taxon 061 strain F0704     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1556573..1571199 1556997..1557299 within 0


Gene organization within MGE regions


Location: 1556573..1571199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW271_RS07620 (HW271_07620) comGF/cglF 1556573..1557034 (-) 462 WP_178895504.1 competence type IV pilus minor pilin ComGF Machinery gene
  HW271_RS07625 (HW271_07625) comGE/cglE 1556997..1557299 (-) 303 WP_178895505.1 competence type IV pilus minor pilin ComGE Machinery gene
  HW271_RS07630 (HW271_07630) comGD/cglD 1557262..1557696 (-) 435 WP_254730736.1 competence type IV pilus minor pilin ComGD Machinery gene
  HW271_RS07635 (HW271_07635) comGC/cglC 1557659..1557982 (-) 324 WP_178895506.1 competence type IV pilus major pilin ComGC Machinery gene
  HW271_RS07640 (HW271_07640) comGB/cglB 1557983..1558999 (-) 1017 WP_178895665.1 competence type IV pilus assembly protein ComGB Machinery gene
  HW271_RS07645 (HW271_07645) comGA/cglA/cilD 1558947..1559888 (-) 942 WP_178895507.1 competence type IV pilus ATPase ComGA Machinery gene
  HW271_RS07650 (HW271_07650) - 1559941..1560318 (-) 378 WP_178895508.1 DUF1033 family protein -
  HW271_RS07655 (HW271_07655) nagA 1560485..1561636 (-) 1152 WP_178895509.1 N-acetylglucosamine-6-phosphate deacetylase -
  HW271_RS07660 (HW271_07660) - 1562219..1562842 (-) 624 WP_178895510.1 hypothetical protein -
  HW271_RS07665 (HW271_07665) - 1562928..1565552 (-) 2625 WP_178895511.1 SEC10/PgrA surface exclusion domain-containing protein -
  HW271_RS07670 (HW271_07670) - 1565704..1566033 (+) 330 WP_049478720.1 helix-turn-helix domain-containing protein -
  HW271_RS07675 (HW271_07675) - 1566125..1566958 (-) 834 WP_178895512.1 hypothetical protein -
  HW271_RS07680 (HW271_07680) - 1567125..1567481 (+) 357 WP_178895513.1 hypothetical protein -
  HW271_RS07685 (HW271_07685) - 1567485..1568645 (+) 1161 WP_178895514.1 cell division protein FtsK -
  HW271_RS07690 (HW271_07690) - 1568840..1569259 (+) 420 WP_259274708.1 hypothetical protein -
  HW271_RS07695 (HW271_07695) - 1569469..1570797 (+) 1329 WP_178895515.1 Rep family protein -
  HW271_RS07700 (HW271_07700) - 1570861..1571199 (+) 339 WP_049478726.1 DUF771 domain-containing protein -

Sequence

Protein


Download         Length: 100 a.a.        Molecular weight: 11278.34 Da        Isoelectric Point: 10.0949

>NTDB_id=463249 HW271_RS07625 WP_178895505.1 1556997..1557299(-) (comGE/cglE) [Streptococcus sp. oral taxon 061 strain F0704]
MERLNVFKKQKIKAVILLEAVISLAIFASIATLLLGQIQESRKREVELLKQEEVLRVARMALQTGQKELTVNGLTVHVVS
NERGLEVYHGTEKLLAIQDK

Nucleotide


Download         Length: 303 bp        

>NTDB_id=463249 HW271_RS07625 WP_178895505.1 1556997..1557299(-) (comGE/cglE) [Streptococcus sp. oral taxon 061 strain F0704]
ATGGAAAGATTAAACGTATTCAAGAAGCAAAAAATTAAGGCGGTTATTTTACTGGAAGCAGTGATTTCACTAGCTATTTT
TGCCAGTATAGCAACTCTCTTACTAGGACAAATTCAAGAAAGTAGAAAAAGAGAAGTAGAACTTCTAAAACAGGAAGAAG
TTTTGCGAGTGGCTCGTATGGCTTTGCAGACTGGACAAAAGGAGTTGACTGTCAATGGCCTTACGGTTCATGTTGTCTCG
AATGAGCGAGGTTTGGAGGTATACCATGGAACGGAGAAATTACTGGCGATTCAAGACAAGTAG

Domains

Predicted by InterproScan.

Type II secretory pathway pseudopilin (ComGE)

(16-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H8ZBB4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGE/cglE Streptococcus pneumoniae D39

66

100

0.66
  comGE/cglE Streptococcus pneumoniae R6

66

100

0.66
  comGE/cglE Streptococcus pneumoniae TIGR4

66

100

0.66
  comGE/cglE Streptococcus pneumoniae Rx1

66

100

0.66
  comGE/cglE Streptococcus mitis SK321

65.657

99

0.65
  comGE/cglE Streptococcus mitis NCTC 12261

65

100

0.65
  comYE Streptococcus mutans UA140

44.318

88

0.39
  comYE Streptococcus mutans UA159

44.318

88

0.39