Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HV088_RS19275 Genome accession   NZ_CP058146
Coordinates   4002410..4003390 (-) Length   326 a.a.
NCBI ID   WP_045623032.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain RHBSTW-00133     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3997410..4008390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV088_RS19245 (HV088_19250) - 3998287..3998784 (+) 498 WP_023298567.1 SprT family zinc-dependent metalloprotease -
  HV088_RS19250 (HV088_19255) endA 3998879..3999586 (+) 708 WP_045623030.1 deoxyribonuclease I -
  HV088_RS19255 (HV088_19260) rsmE 3999638..4000369 (+) 732 WP_003860033.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HV088_RS19260 (HV088_19265) gshB 4000401..4001348 (+) 948 WP_003860032.1 glutathione synthase -
  HV088_RS19265 (HV088_19270) - 4001423..4001983 (+) 561 WP_045623031.1 YqgE/AlgH family protein -
  HV088_RS19270 (HV088_19275) ruvX 4001983..4002399 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  HV088_RS19275 (HV088_19280) pilT 4002410..4003390 (-) 981 WP_045623032.1 type IV pilus twitching motility protein PilT Machinery gene
  HV088_RS19280 (HV088_19285) - 4003408..4004109 (+) 702 WP_045623033.1 YggS family pyridoxal phosphate-dependent enzyme -
  HV088_RS19285 (HV088_19290) - 4004131..4004697 (+) 567 WP_003860024.1 YggT family protein -
  HV088_RS19290 (HV088_19295) yggU 4004694..4004990 (+) 297 WP_022649356.1 DUF167 family protein YggU -
  HV088_RS19295 (HV088_19300) - 4004994..4005587 (+) 594 WP_022649357.1 XTP/dITP diphosphatase -
  HV088_RS19300 (HV088_19305) hemW 4005580..4006728 (+) 1149 WP_023306915.1 radical SAM family heme chaperone HemW -
  HV088_RS19305 (HV088_19310) - 4006785..4007123 (+) 339 WP_023298571.1 endonuclease domain-containing protein -
  HV088_RS19310 (HV088_19315) - 4007239..4007955 (-) 717 WP_022649360.1 DUF2884 domain-containing protein -
  HV088_RS19315 (HV088_19320) - 4008013..4008339 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35746.71 Da        Isoelectric Point: 6.3417

>NTDB_id=462323 HV088_RS19275 WP_045623032.1 4002410..4003390(-) (pilT) [Enterobacter hormaechei strain RHBSTW-00133]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSERCLIRQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQERL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=462323 HV088_RS19275 WP_045623032.1 4002410..4003390(-) (pilT) [Enterobacter hormaechei strain RHBSTW-00133]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCCGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAGCAGCAGGGCGCCTGGTGGGCAAACGGGCAGGTAGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTACGCCTGTTGCCGCTGACGTGTCCGCAGCTCTCTGCGTTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACTGGCGCTACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTTCTCAATCACCATACGGACGGGCACATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTCATCCGGCAGCGGGAGATAGGCCAGCATAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGTGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCTGAGACTGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGATACC
TTCCCGGCGCAGGAGAAAGATCCGGTTCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTACTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGTA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGAGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.411

97.853

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.411

97.853

0.503
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Pseudomonas stutzeri DSM 10701

48.438

98.16

0.475
  pilT Neisseria meningitidis 8013

47.975

98.466

0.472
  pilT Acinetobacter baylyi ADP1

46.933

100

0.469
  pilT Neisseria gonorrhoeae MS11

47.664

98.466

0.469
  pilT Acinetobacter baumannii strain A118

47.5

98.16

0.466
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Acinetobacter baumannii D1279779

47.5

98.16

0.466
  pilT Pseudomonas aeruginosa PAK

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.226

100

0.383
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38