Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   JNN40_RS19165 Genome accession   NZ_CP068802
Coordinates   4012418..4013911 (+) Length   497 a.a.
NCBI ID   WP_094931624.1    Uniprot ID   -
Organism   Escherichia coli strain RIVM_C018249     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4007418..4018911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNN40_RS19145 (JNN40_19145) pilD 4008066..4008875 (+) 810 WP_029305424.1 prepilin peptidase PppA Machinery gene
  JNN40_RS19150 (JNN40_19150) gspS2 4008941..4009351 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  JNN40_RS19155 (JNN40_19155) gspC 4009369..4010328 (+) 960 WP_000135091.1 type II secretion system protein GspC -
  JNN40_RS19160 (JNN40_19160) gspD 4010358..4012418 (+) 2061 WP_000498829.1 type II secretion system secretin GspD -
  JNN40_RS19165 (JNN40_19165) pilB/pilB1 4012418..4013911 (+) 1494 WP_094931624.1 type II secretion system ATPase GspE Machinery gene
  JNN40_RS19170 (JNN40_19170) gspF 4013911..4015134 (+) 1224 WP_024242170.1 type II secretion system inner membrane protein GspF -
  JNN40_RS19175 (JNN40_19175) gspG 4015151..4015606 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  JNN40_RS19180 (JNN40_19180) gspH 4015610..4016173 (+) 564 WP_094931626.1 type II secretion system minor pseudopilin GspH -
  JNN40_RS19185 (JNN40_19185) gspI 4016170..4016541 (+) 372 WP_000820090.1 type II secretion system minor pseudopilin GspI -
  JNN40_RS19190 (JNN40_19190) gspJ 4016538..4017143 (+) 606 WP_029305420.1 type II secretion system minor pseudopilin GspJ -
  JNN40_RS19195 (JNN40_19195) gspK 4017140..4018117 (+) 978 WP_029305419.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55234.36 Da        Isoelectric Point: 5.9800

>NTDB_id=461545 JNN40_RS19165 WP_094931624.1 4012418..4013911(+) (pilB/pilB1) [Escherichia coli strain RIVM_C018249]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNALFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLNHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCSDCRQAAPA
TDEEKRLMGISDTHAVTLYHPQGCPACNHKGFRGRTVIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=461545 JNN40_RS19165 WP_094931624.1 4012418..4013911(+) (pilB/pilB1) [Escherichia coli strain RIVM_C018249]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCTCTCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGTGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGGCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCATCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTGAACCATGAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGTATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACATTCGCCCGTGGCCTGCGCGCAATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTCCGCACGCTGTGCTCCGACTGCCGTCAGGCCGCGCCTGCC
ACCGACGAAGAAAAACGTCTTATGGGGATCTCCGATACGCATGCCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGTCATCCATGAACTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

40.111

100

0.437
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.927

99.396

0.437
  pilB Vibrio parahaemolyticus RIMD 2210633

47.859

79.879

0.382
  pilB Acinetobacter baumannii D1279779

46.867

80.282

0.376
  pilB Legionella pneumophila strain ERS1305867

43.387

86.72

0.376
  pilB Vibrio campbellii strain DS40M4

46.97

79.678

0.374
  pilB Vibrio cholerae strain A1552

46.465

79.678

0.37