Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   JKV45_RS04055 Genome accession   NZ_CP068678
Coordinates   866034..866657 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain NCCP15783     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 861034..871657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JKV45_RS04035 (JKV45_04030) aroB 861627..862733 (-) 1107 WP_019726985.1 3-dehydroquinate synthase -
  JKV45_RS04040 (JKV45_04035) aroK 862783..863301 (-) 519 WP_003095833.1 shikimate kinase AroK -
  JKV45_RS04045 (JKV45_04040) pilQ 863313..865459 (-) 2147 Protein_798 type 4a pilus secretin PilQ -
  JKV45_RS04050 (JKV45_04045) comL/pilP 865513..866037 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  JKV45_RS04055 (JKV45_04050) pilO 866034..866657 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  JKV45_RS04060 (JKV45_04055) pilN 866654..867250 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  JKV45_RS04065 (JKV45_04060) pilM 867250..868314 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  JKV45_RS04070 (JKV45_04065) - 868499..870970 (+) 2472 WP_019726988.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=460494 JKV45_RS04055 WP_003103268.1 866034..866657(-) (pilO) [Pseudomonas aeruginosa strain NCCP15783]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=460494 JKV45_RS04055 WP_003103268.1 866034..866657(-) (pilO) [Pseudomonas aeruginosa strain NCCP15783]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401