Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HV333_RS04220 Genome accession   NZ_CP056235
Coordinates   847443..848423 (+) Length   326 a.a.
NCBI ID   WP_103848972.1    Uniprot ID   -
Organism   Citrobacter freundii strain RHBSTW-00942     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 842443..853423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV333_RS04190 (HV333_04190) ansB 842957..844003 (+) 1047 WP_003027108.1 L-asparaginase 2 -
  HV333_RS04195 (HV333_04195) hemW 844119..845255 (-) 1137 WP_103848974.1 radical SAM family heme chaperone HemW -
  HV333_RS04200 (HV333_04200) - 845248..845841 (-) 594 WP_003027104.1 XTP/dITP diphosphatase -
  HV333_RS04205 (HV333_04205) yggU 845849..846139 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  HV333_RS04210 (HV333_04210) - 846136..846702 (-) 567 WP_003825417.1 YggT family protein -
  HV333_RS04215 (HV333_04215) - 846721..847425 (-) 705 WP_103848973.1 YggS family pyridoxal phosphate-dependent enzyme -
  HV333_RS04220 (HV333_04220) pilT 847443..848423 (+) 981 WP_103848972.1 type IV pilus twitching motility protein PilT Machinery gene
  HV333_RS04225 (HV333_04225) ruvX 848420..848836 (-) 417 WP_003027087.1 Holliday junction resolvase RuvX -
  HV333_RS04230 (HV333_04230) - 848836..849399 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  HV333_RS04235 (HV333_04235) gshB 849575..850522 (-) 948 WP_003027083.1 glutathione synthase -
  HV333_RS04240 (HV333_04240) rsmE 850542..851273 (-) 732 WP_003027080.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HV333_RS04245 (HV333_04245) endA 851348..852055 (-) 708 WP_103848971.1 deoxyribonuclease I -
  HV333_RS04250 (HV333_04250) - 852150..852647 (-) 498 WP_003838223.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35985.06 Da        Isoelectric Point: 6.4255

>NTDB_id=458822 HV333_RS04220 WP_103848972.1 847443..848423(+) (pilT) [Citrobacter freundii strain RHBSTW-00942]
MNMEEIVALSVKHNVSDLHLCNAWPARWRRRGKVESAPFTTPDVENLLMCWLSEQQQVQLQEQGQIDFAVTLTDSRRLRT
SAFVHQQGTSLALRLLPLDCPHLDDLQPPAVIPELLHSENGLILVTGATGSGKSTTLAAMVEYLNQHIEGHILTLEDPIE
YRYTSRRCLIQQREVGAHCASFAAGLRGVLREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDS
FAATEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=458822 HV333_RS04220 WP_103848972.1 847443..848423(+) (pilT) [Citrobacter freundii strain RHBSTW-00942]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCCGCGCG
TTGGCGCAGGCGTGGAAAAGTCGAAAGCGCACCGTTTACCACGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTTGCAGGAGCAAGGGCAGATTGATTTTGCCGTTACCCTGACGGACTCCCGGCGGCTGCGCACC
AGCGCATTTGTCCATCAGCAGGGAACCTCGCTGGCGCTAAGACTGCTACCGCTGGATTGTCCTCATTTAGACGATCTTCA
GCCTCCCGCGGTCATACCTGAACTGCTTCACAGTGAAAATGGGTTGATTCTGGTGACAGGCGCTACCGGCAGCGGTAAAT
CTACGACCCTGGCGGCGATGGTGGAGTATCTTAATCAGCATATTGAGGGGCACATTCTGACGCTGGAAGATCCTATTGAA
TATCGCTACACCAGCCGACGTTGTCTGATTCAACAGCGGGAGGTGGGCGCACACTGCGCCTCTTTCGCCGCCGGTTTGCG
CGGTGTGCTACGCGAAGATCCCGACGTTATTTTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGGCTGGCATTAACGG
CGGCGGAAACCGGACATCTGGTGCTGGCAACGTTACATACGCGAGGTGCGGCGCAGGCCATCGCGCGGCTGGTGGATTCC
TTTGCAGCAACAGAGAAAGATCCTGTGCGTAACCAACTGGCAGACAGCCTGCGGGCGGTTCTTTCGCAAAAACTTGAGGA
GGATAAGCAGGGGGGACGCGTGGCGCTATTCGAACTACTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGCGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACATTTGCACAAAGTTTGCAGCAGCGA
CAGGCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

46.341

100

0.466
  pilT Neisseria gonorrhoeae MS11

46.037

100

0.463
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Legionella pneumophila strain ERS1305867

45.652

98.773

0.451
  pilT Legionella pneumophila strain Lp02

45.652

98.773

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.009

97.239

0.399
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365