Detailed information NTG0458411

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HV312_RS21650
• Genome accession: NZ_CP056145
• Coordinates: 4477687..4478487 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola strain RHBSTW-00883
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4472687..4483487

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HV312_RS21615 (HV312_21615)	gspH	4472840..4473355 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
HV312_RS21620 (HV312_21620)	gspI	4473352..4473717 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
HV312_RS21625 (HV312_21625)	gspJ	4473714..4474310 (+)	597	WP_032731630.1	type II secretion system minor pseudopilin GspJ	-
HV312_RS21630 (HV312_21630)	gspK	4474307..4475287 (+)	981	WP_077264701.1	type II secretion system minor pseudopilin GspK	-
HV312_RS21635 (HV312_21635)	gspL	4475280..4476476 (+)	1197	WP_087731211.1	type II secretion system protein GspL	-
HV312_RS21640 (HV312_21640)	-	4476469..4476954 (+)	486	WP_012968921.1	type II secretion system protein M	-
HV312_RS21645 (HV312_21645)	-	4476944..4477690 (+)	747	WP_016160310.1	type II secretion system protein N	-
HV312_RS21650 (HV312_21650)	pilD	4477687..4478487 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
HV312_RS21655 (HV312_21655)	hrpB	4478510..4480939 (-)	2430	WP_088717607.1	ATP-dependent helicase HrpB	-
HV312_RS21660 (HV312_21660)	thpR	4481012..4481548 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
HV312_RS21665 (HV312_21665)	sfsA	4481548..4482264 (+)	717	WP_022066362.1	DNA/RNA nuclease SfsA	-
HV312_RS21670 (HV312_21670)	dksA	4482427..4482882 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=458411 HV312_RS21650 WP_008807384.1 4477687..4478487(+) (pilD) [Klebsiella variicola strain RHBSTW-00883]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=458411 HV312_RS21650 WP_008807384.1 4477687..4478487(+) (pilD) [Klebsiella variicola strain RHBSTW-00883]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425