Detailed information NTG0045825

Bioinformatically predicted

Overview

• Name: pilA/pilA1
• Type: Machinery gene
• Locus tag: TCCBUS3UF1_RS04295
• Genome accession: NC_017278
• Coordinates: 854773..855243 (-)
• Length: 156 a.a.
• NCBI ID: WP_014515281.1
• Uniprot ID: -
• Organism: Thermus sp. CCB_US3_UF1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 849773..860243

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
TCCBUS3UF1_RS04265 (TCCBUS3UF1_8670)	-	850263..850547 (-)	285	WP_050802035.1	hypothetical protein	-
TCCBUS3UF1_RS12425	-	850566..850616 (-)	51	Protein_858	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
TCCBUS3UF1_RS04270 (TCCBUS3UF1_8680)	-	850686..851045 (-)	360	WP_014515275.1	type II secretion system protein	-
TCCBUS3UF1_RS04275 (TCCBUS3UF1_8700)	comZ	851262..852929 (-)	1668	WP_014515277.1	pilus assembly PilX N-terminal domain-containing protein	Machinery gene
TCCBUS3UF1_RS04280 (TCCBUS3UF1_8710)	pilA/pilA3	852939..853643 (-)	705	WP_014515278.1	PilW family protein	Machinery gene
TCCBUS3UF1_RS04285 (TCCBUS3UF1_8720)	pilA/pilA2	853640..854248 (-)	609	WP_014515279.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
TCCBUS3UF1_RS04290 (TCCBUS3UF1_8730)	-	854245..854718 (-)	474	WP_041433756.1	GspH/FimT family protein	-
TCCBUS3UF1_RS04295 (TCCBUS3UF1_8740)	pilA/pilA1	854773..855243 (-)	471	WP_014515281.1	GspH/FimT family protein	Machinery gene
TCCBUS3UF1_RS04300 (TCCBUS3UF1_8750)	-	855310..856500 (+)	1191	WP_014515282.1	thiolase family protein	-
TCCBUS3UF1_RS04305	-	856497..856805 (-)	309	WP_041433963.1	DUF503 domain-containing protein	-
TCCBUS3UF1_RS04310 (TCCBUS3UF1_8770)	-	857092..859557 (-)	2466	WP_041433964.1	outer membrane protein assembly factor	-

Sequence

Protein

• Length: 156 a.a.
• Molecular weight: 16682.39 Da
• Isoelectric Point: 11.5020

>NTDB_id=45825 TCCBUS3UF1_RS04295 WP_014515281.1 854773..855243(-) (pilA/pilA1) [Thermus sp. CCB_US3_UF1]
MRQPGFSLLELLLVLGLLGVLLGLGLPLLSPNRLALNGAAQTLAAQVHRTRLEAIRHNAFAGLHVFTEGAGGYVVFLDRD
GDRRPTPGEELQTVRFGEGAWARVRLDPGQSALGNLPLLFDPRGIPAKPITATIALTSGPATRKVILSQQGRPRLD

Nucleotide

• Length: 471 bp

>NTDB_id=45825 TCCBUS3UF1_RS04295 WP_014515281.1 854773..855243(-) (pilA/pilA1) [Thermus sp. CCB_US3_UF1]
ATGCGGCAACCGGGGTTTAGCCTGCTGGAGCTTCTCCTGGTTCTGGGCCTTTTGGGGGTCCTCCTTGGCCTGGGCCTACC
CCTCCTCTCCCCCAACCGCCTGGCCCTGAACGGGGCAGCCCAGACCCTGGCCGCCCAGGTCCACAGGACGCGGCTGGAGG
CCATCCGCCATAACGCCTTCGCCGGGCTCCACGTGTTCACGGAAGGGGCGGGAGGGTACGTGGTCTTCCTGGACCGGGAC
GGGGACCGCCGCCCCACGCCAGGGGAGGAGCTCCAGACCGTGCGCTTCGGGGAAGGGGCCTGGGCCCGGGTGCGGCTGGA
CCCCGGGCAGAGCGCCCTCGGCAACCTTCCCCTCCTCTTCGACCCCCGCGGCATCCCCGCCAAGCCCATCACCGCCACCA
TCGCCCTCACTTCTGGTCCGGCCACCCGCAAGGTGATCCTCAGCCAGCAGGGCCGCCCCCGCCTGGACTAA

Domains

Predicted by InterproScan.

• Type II transport protein GspH (GspH) (39-151)

• Prokaryotic N-terminal methylation motif (N_methyl) (2-24)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA/pilA1	Thermus thermophilus HB27	71.654	81.41	0.583