Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA3   Type   Machinery gene
Locus tag   TCCBUS3UF1_RS04280 Genome accession   NC_017278
Coordinates   852939..853643 (-) Length   234 a.a.
NCBI ID   WP_014515278.1    Uniprot ID   -
Organism   Thermus sp. CCB_US3_UF1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 847939..858643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TCCBUS3UF1_RS04260 (TCCBUS3UF1_8660) - 848674..850254 (-) 1581 WP_014515273.1 O-antigen ligase family protein -
  TCCBUS3UF1_RS04265 (TCCBUS3UF1_8670) - 850263..850547 (-) 285 WP_050802035.1 hypothetical protein -
  TCCBUS3UF1_RS12425 - 850566..850616 (-) 51 Protein_858 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  TCCBUS3UF1_RS04270 (TCCBUS3UF1_8680) - 850686..851045 (-) 360 WP_014515275.1 type II secretion system protein -
  TCCBUS3UF1_RS04275 (TCCBUS3UF1_8700) comZ 851262..852929 (-) 1668 WP_014515277.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  TCCBUS3UF1_RS04280 (TCCBUS3UF1_8710) pilA/pilA3 852939..853643 (-) 705 WP_014515278.1 PilW family protein Machinery gene
  TCCBUS3UF1_RS04285 (TCCBUS3UF1_8720) pilA/pilA2 853640..854248 (-) 609 WP_014515279.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  TCCBUS3UF1_RS04290 (TCCBUS3UF1_8730) - 854245..854718 (-) 474 WP_041433756.1 GspH/FimT family protein -
  TCCBUS3UF1_RS04295 (TCCBUS3UF1_8740) pilA/pilA1 854773..855243 (-) 471 WP_014515281.1 GspH/FimT family protein Machinery gene
  TCCBUS3UF1_RS04300 (TCCBUS3UF1_8750) - 855310..856500 (+) 1191 WP_014515282.1 thiolase family protein -
  TCCBUS3UF1_RS04305 - 856497..856805 (-) 309 WP_041433963.1 DUF503 domain-containing protein -

Sequence

Protein


Download         Length: 234 a.a.        Molecular weight: 24822.35 Da        Isoelectric Point: 8.2143

>NTDB_id=45823 TCCBUS3UF1_RS04280 WP_014515278.1 852939..853643(-) (pilA/pilA3) [Thermus sp. CCB_US3_UF1]
MKRKGLTLVEVLVAAAILVVLLGVAVRYFASTAELGRNTQAKSELQDRVRMVMQVATGDLQMAGARYWNAGTANRAFTLP
LGKVLLGTDGGAKDTLSLYYVTSLRGQNEACRRVGYDFQGDTLRRSDVNATPTTGNDCTEPASSPQPLAEGILALDIQYV
CSDGTAKNTPDCGAAAYPRSAIVEVVGYTLSPVQGPGPATLTTHTGQSVSCPQGRACYALRQEVLMPNLKPLPQ

Nucleotide


Download         Length: 705 bp        

>NTDB_id=45823 TCCBUS3UF1_RS04280 WP_014515278.1 852939..853643(-) (pilA/pilA3) [Thermus sp. CCB_US3_UF1]
GTGAAGCGCAAGGGGCTCACCCTGGTGGAAGTCCTGGTGGCCGCGGCCATCCTGGTGGTCCTCCTGGGGGTGGCGGTGCG
GTACTTCGCCAGCACCGCCGAGCTCGGGCGGAATACCCAGGCCAAAAGCGAACTCCAGGACCGGGTGCGGATGGTGATGC
AGGTGGCAACCGGGGACCTGCAGATGGCCGGGGCCCGCTACTGGAACGCGGGCACCGCCAACCGGGCCTTCACCCTGCCC
CTAGGCAAGGTACTCCTGGGCACCGATGGCGGGGCCAAGGACACCCTTAGCCTCTACTACGTCACCAGCCTGCGGGGCCA
GAACGAGGCCTGCCGCCGGGTGGGCTACGACTTCCAGGGCGACACCCTGCGGCGAAGCGACGTGAACGCCACCCCTACCA
CAGGCAACGACTGCACCGAACCCGCCTCCAGCCCCCAGCCCCTGGCCGAGGGTATCCTGGCCCTGGACATCCAGTACGTG
TGCAGCGACGGCACCGCCAAGAACACCCCCGACTGCGGCGCCGCCGCCTACCCCCGTTCGGCCATCGTGGAGGTGGTGGG
ATACACCCTAAGCCCCGTGCAAGGCCCCGGCCCCGCCACCCTCACCACCCACACCGGGCAATCGGTTTCCTGCCCCCAGG
GCAGGGCCTGCTACGCCCTGAGGCAGGAGGTCTTGATGCCCAACCTCAAGCCCTTGCCCCAATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA3 Thermus thermophilus HB27

72.727

98.718

0.718

Multiple sequence alignment