Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   TCCBUS3UF1_RS03095 Genome accession   NC_017278
Coordinates   621176..622309 (-) Length   377 a.a.
NCBI ID   WP_014515044.1    Uniprot ID   -
Organism   Thermus sp. CCB_US3_UF1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 616176..627309
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TCCBUS3UF1_RS03075 (TCCBUS3UF1_6320) pilQ 616740..618998 (-) 2259 WP_041433717.1 secretin N-terminal domain-containing protein Machinery gene
  TCCBUS3UF1_RS12010 - 619010..619591 (-) 582 WP_231291413.1 hypothetical protein -
  TCCBUS3UF1_RS03085 (TCCBUS3UF1_6340) pilO 619971..620570 (-) 600 WP_014515042.1 type 4a pilus biogenesis protein PilO Machinery gene
  TCCBUS3UF1_RS03090 (TCCBUS3UF1_6350) pilN 620560..621183 (-) 624 WP_014515043.1 competence protein Machinery gene
  TCCBUS3UF1_RS03095 (TCCBUS3UF1_6360) pilM 621176..622309 (-) 1134 WP_014515044.1 type IV pilus assembly protein PilM Machinery gene
  TCCBUS3UF1_RS03100 (TCCBUS3UF1_6370) - 622472..623476 (-) 1005 WP_014515045.1 homoisocitrate dehydrogenase -
  TCCBUS3UF1_RS03105 (TCCBUS3UF1_6380) - 623545..624039 (-) 495 WP_014515046.1 DUF5317 domain-containing protein -
  TCCBUS3UF1_RS03110 (TCCBUS3UF1_6390) - 624090..625520 (-) 1431 WP_014515047.1 HD-GYP domain-containing protein -
  TCCBUS3UF1_RS03125 (TCCBUS3UF1_6400) - 626430..626795 (+) 366 WP_014515048.1 hypothetical protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41182.59 Da        Isoelectric Point: 4.5069

>NTDB_id=45819 TCCBUS3UF1_RS03095 WP_014515044.1 621176..622309(-) (pilM) [Thermus sp. CCB_US3_UF1]
MVSGFSKLFKPRVEALGLEIGASSLKLVELSGHPPALRAFATRPTPPGMLAEGVVAEPAALAQEIRELLAEARTRKRYVV
SAVPNPSVILRTIQVPKMPPKEMEEAVRWEAERYIPFPIDEVVLDFAPLEPLSEVSEGEQTEVMVGVARQEAVAGLLEAL
RGAGLMPVVLDVKPFAGLYPLEAELGAEPERVAVAVEIGAESTSLVLTRGDRPLAVRVLTLSGKDFTEAIGKAFGLDFLT
AEEVKRTYGLATIPTEDEELLLDFDAERERYSPARIYDAIRPILVDLTQEIRRSLEFFRVQLGDIHPEVGYLYGGGSRLR
GLATLLTDTLGVSFTTPDPWQGIQVDPKRFDLEKLKELGPELMVPIGLALRGVSPLD

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=45819 TCCBUS3UF1_RS03095 WP_014515044.1 621176..622309(-) (pilM) [Thermus sp. CCB_US3_UF1]
GTGGTTTCGGGTTTCAGCAAGCTATTCAAGCCTCGCGTGGAGGCCCTGGGGCTGGAGATCGGGGCGTCGAGCCTCAAGCT
GGTGGAGCTTTCCGGCCATCCTCCCGCCCTGCGAGCCTTCGCCACCCGCCCCACCCCACCGGGGATGCTGGCGGAAGGGG
TGGTGGCTGAGCCCGCTGCCTTGGCCCAGGAGATTCGGGAACTCCTGGCCGAAGCCCGCACCCGCAAGCGCTACGTGGTG
AGCGCCGTCCCCAACCCCAGCGTGATCCTGCGTACCATCCAGGTGCCCAAGATGCCGCCCAAGGAGATGGAGGAGGCGGT
CCGCTGGGAAGCCGAACGCTACATCCCCTTCCCCATAGATGAGGTGGTCTTGGACTTCGCCCCCCTCGAGCCCCTCTCCG
AGGTTTCCGAGGGGGAGCAGACCGAGGTGATGGTTGGGGTGGCGCGGCAGGAAGCGGTGGCCGGGCTCCTGGAGGCCTTG
CGGGGGGCCGGCCTGATGCCGGTGGTCCTAGACGTAAAGCCCTTCGCCGGCCTCTACCCCTTGGAAGCCGAGCTCGGCGC
CGAGCCCGAGCGGGTGGCGGTGGCGGTGGAGATCGGGGCCGAGAGCACCAGCCTGGTCCTGACCCGGGGGGACCGGCCCC
TGGCGGTGCGGGTGCTCACCCTTTCGGGCAAGGATTTCACCGAGGCCATCGGCAAGGCCTTCGGCCTGGACTTCCTCACC
GCCGAGGAGGTCAAGCGCACCTATGGCCTGGCCACCATCCCCACCGAGGACGAGGAGCTCCTTTTGGACTTTGACGCCGA
ACGGGAGCGCTATAGCCCCGCCCGCATCTACGACGCCATCCGCCCCATCTTGGTGGACCTCACCCAGGAGATCCGAAGAA
GCCTGGAGTTTTTCCGGGTGCAGCTGGGGGATATCCACCCTGAGGTGGGCTACCTCTACGGGGGAGGTAGCCGCCTGCGG
GGCCTGGCCACCCTGCTCACCGATACCCTGGGGGTCAGCTTCACCACCCCAGACCCCTGGCAGGGCATCCAGGTGGACCC
CAAGCGCTTTGACCTGGAAAAGCTAAAGGAGCTGGGCCCCGAGCTGATGGTGCCCATAGGCCTGGCCCTAAGGGGGGTGA
GTCCCCTTGATTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-372)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Thermus thermophilus HB27

77.984

100

0.78

Multiple sequence alignment