Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA3   Type   Machinery gene
Locus tag   THTHE16_RS06280 Genome accession   NC_017272
Coordinates   1184996..1185700 (+) Length   234 a.a.
NCBI ID   WP_014510473.1    Uniprot ID   F6DHL6
Organism   Thermus thermophilus SG0.5JP17-16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1179996..1190700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THTHE16_RS06255 (Ththe16_1227) ilvB 1180464..1182152 (-) 1689 WP_014510469.1 biosynthetic-type acetolactate synthase large subunit -
  THTHE16_RS06260 (Ththe16_1228) - 1182308..1183288 (-) 981 WP_008631297.1 YpdA family putative bacillithiol disulfide reductase -
  THTHE16_RS06265 (Ththe16_1229) pilA/pilA1 1183446..1183916 (+) 471 WP_014510470.1 GspH/FimT family protein Machinery gene
  THTHE16_RS06270 (Ththe16_1230) - 1183972..1184445 (+) 474 WP_014510471.1 GspH/FimT family pseudopilin -
  THTHE16_RS06275 (Ththe16_1231) pilA/pilA2 1184442..1184999 (+) 558 WP_014510472.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  THTHE16_RS06280 (Ththe16_1232) pilA/pilA3 1184996..1185700 (+) 705 WP_014510473.1 PilW family protein Machinery gene
  THTHE16_RS06285 (Ththe16_1233) comZ 1185711..1187426 (+) 1716 WP_014510474.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  THTHE16_RS12275 (Ththe16_1234) - 1187666..1188073 (+) 408 WP_014510475.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  THTHE16_RS06295 (Ththe16_1235) - 1188242..1188439 (+) 198 WP_018461701.1 nucleotidyltransferase family protein -
  THTHE16_RS06300 (Ththe16_1236) - 1188610..1189089 (+) 480 WP_014510476.1 DUF1640 domain-containing protein -
  THTHE16_RS06305 (Ththe16_1237) - 1189165..1189707 (+) 543 WP_008631313.1 Uma2 family endonuclease -
  THTHE16_RS06310 (Ththe16_1238) - 1189730..1190029 (+) 300 WP_008631315.1 nucleotidyltransferase family protein -

Sequence

Protein


Download         Length: 234 a.a.        Molecular weight: 24999.42 Da        Isoelectric Point: 7.4626

>NTDB_id=45780 THTHE16_RS06280 WP_014510473.1 1184996..1185700(+) (pilA/pilA3) [Thermus thermophilus SG0.5JP17-16]
MKRRGFTLIEVLVAGAILVTVLTVAIRYFASTAELGRETQAKSELQDRVRMVMQVVTGDLQMAGARYWNSGNQNRAFSLP
LPPLSGSDGGPKDTLTLYYVTSLRDLASACRRVDYGFDGDTLRRSDVNATPSSGSDCTTPSPNLQPLAEGILALDIQYQC
SDGSQKDSPDCGTDAYLRSAKVTVAGYSLTPVTNPGPASLTTVTGKTLACPQGRACYALTQEVLMPNLKPLPTP

Nucleotide


Download         Length: 705 bp        

>NTDB_id=45780 THTHE16_RS06280 WP_014510473.1 1184996..1185700(+) (pilA/pilA3) [Thermus thermophilus SG0.5JP17-16]
ATGAAGCGCCGAGGCTTTACCCTGATAGAGGTCCTTGTGGCCGGGGCCATCCTGGTCACGGTCCTGACCGTGGCCATCCG
CTACTTCGCCAGCACGGCGGAGCTCGGCCGGGAAACCCAGGCCAAGAGCGAGCTTCAGGACCGGGTGCGGATGGTGATGC
AGGTGGTCACCGGGGACCTCCAGATGGCGGGGGCCCGCTACTGGAATAGCGGCAACCAAAACCGGGCCTTCAGCCTCCCC
CTTCCCCCGCTTTCGGGAAGCGACGGAGGCCCTAAGGACACCCTAACCCTCTACTACGTCACGAGCCTGCGGGACCTGGC
CTCCGCCTGCCGCCGGGTGGACTACGGGTTTGACGGGGACACCCTGCGCCGGAGCGACGTGAACGCCACCCCCTCTTCGG
GAAGCGATTGCACCACCCCCTCTCCGAACTTGCAGCCCTTGGCCGAAGGGATCTTGGCCCTGGACATCCAATACCAGTGC
AGCGACGGGAGCCAGAAGGACTCCCCCGACTGTGGTACCGATGCCTACCTCCGCTCGGCCAAGGTCACGGTGGCGGGCTA
CTCCCTGACCCCGGTGACCAACCCGGGCCCCGCCTCCCTCACCACCGTCACCGGAAAGACCCTCGCCTGCCCCCAGGGCC
GGGCCTGCTACGCCCTCACCCAGGAGGTCCTCATGCCCAACCTCAAGCCCCTGCCCACCCCCTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F6DHL6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA3 Thermus thermophilus HB27

90.086

99.145

0.893

Multiple sequence alignment