Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HUK62_RS16425 Genome accession   NZ_CP055277
Coordinates   3369829..3370566 (-) Length   245 a.a.
NCBI ID   WP_005401353.1    Uniprot ID   A0A7H8SJL8
Organism   Acinetobacter sp. NEB 394     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3364829..3375566
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK62_RS19140 (HUK62_16400) - 3365625..3366107 (-) 483 WP_265937476.1 shikimate kinase -
  HUK62_RS16405 (HUK62_16405) pilQ 3366196..3367458 (-) 1263 Protein_3193 type IV pilus secretin PilQ -
  HUK62_RS16410 (HUK62_16410) - 3367530..3368480 (+) 951 WP_006582413.1 IS481 family transposase -
  HUK62_RS16415 (HUK62_16415) - 3368491..3369237 (-) 747 Protein_3195 AMIN domain-containing protein -
  HUK62_RS16420 (HUK62_16420) pilP 3369302..3369829 (-) 528 WP_005401352.1 pilus assembly protein PilP Machinery gene
  HUK62_RS16425 (HUK62_16425) pilO 3369829..3370566 (-) 738 WP_005401353.1 type 4a pilus biogenesis protein PilO Machinery gene
  HUK62_RS16430 (HUK62_16430) pilN 3370563..3371219 (-) 657 WP_005401354.1 PilN domain-containing protein Machinery gene
  HUK62_RS16435 (HUK62_16435) - 3371219..3372277 (-) 1059 Protein_3199 pilus assembly protein PilM -
  HUK62_RS16440 (HUK62_16440) ponA 3372444..3374972 (+) 2529 WP_005401356.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 27705.26 Da        Isoelectric Point: 5.1834

>NTDB_id=457393 HUK62_RS16425 WP_005401353.1 3369829..3370566(-) (pilO) [Acinetobacter sp. NEB 394]
MSRDEFEELTSENHAVAKKKMTVDKFFQQFNTLDPNNYGSWPFSVKLTCWIFIILVIGALGYFVVIKPKLDAISNAHAQE
QNLLNEFREKDSKLRNLQQYQVQLQEMEANFNQQLEQLPKETEIPGLVEDINVSGVNSGLKFKNIRLEPEIRQEFFIEQP
ISIEATGDYHSFGSFVSGIAALPRIVTLHDFDISASSNNDKKTDIPQISYSVKAKTYRYVGATATTDVDVKDTKNDKKAD
AGGAK

Nucleotide


Download         Length: 738 bp        

>NTDB_id=457393 HUK62_RS16425 WP_005401353.1 3369829..3370566(-) (pilO) [Acinetobacter sp. NEB 394]
ATGAGTCGCGATGAATTTGAAGAATTAACATCAGAAAACCATGCTGTAGCCAAGAAAAAAATGACGGTTGATAAATTTTT
TCAACAGTTTAATACCTTAGATCCAAACAATTACGGTAGTTGGCCCTTTTCAGTCAAATTGACTTGTTGGATCTTTATCA
TTTTAGTGATTGGGGCATTGGGTTATTTTGTCGTGATTAAGCCGAAGCTTGATGCGATTTCAAATGCACATGCACAAGAA
CAAAATTTACTGAATGAGTTTCGTGAAAAAGATTCAAAATTACGTAATTTGCAGCAATATCAAGTGCAATTGCAAGAAAT
GGAAGCCAATTTTAATCAGCAGTTAGAACAGCTGCCGAAAGAAACTGAAATTCCTGGCTTGGTTGAAGATATCAACGTGA
GCGGCGTAAATTCTGGATTAAAGTTTAAAAATATTCGTTTAGAACCAGAAATTCGCCAAGAGTTCTTTATCGAACAGCCG
ATTTCCATTGAAGCGACGGGTGATTATCATTCTTTTGGTTCATTTGTCAGTGGAATTGCAGCTTTACCGCGTATTGTTAC
CTTGCATGACTTCGATATCTCAGCATCAAGTAACAATGATAAAAAGACGGATATTCCGCAAATTTCATATAGTGTAAAAG
CGAAAACCTACCGTTATGTCGGGGCAACGGCTACAACGGATGTTGATGTAAAAGATACAAAAAATGATAAGAAAGCCGAT
GCGGGAGGTGCCAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H8SJL8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

74.89

92.653

0.694
  comO Acinetobacter baylyi ADP1

72.398

90.204

0.653