Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   JH656_RS04955 Genome accession   NZ_CP066839
Coordinates   1011972..1012631 (-) Length   219 a.a.
NCBI ID   WP_139560537.1    Uniprot ID   -
Organism   Escherichia coli strain Ec-RL2-1X     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1006972..1017631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH656_RS04930 (JH656_04870) parE 1007063..1008955 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  JH656_RS04935 (JH656_04875) ygiN 1009003..1009317 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  JH656_RS04940 (JH656_04880) mdaB 1009348..1009929 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  JH656_RS04945 (JH656_04885) ygiZ 1010248..1010580 (+) 333 WP_000917684.1 DUF2645 family protein -
  JH656_RS04950 (JH656_04890) qseC 1010626..1011975 (-) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  JH656_RS04955 (JH656_04895) ciaR 1011972..1012631 (-) 660 WP_139560537.1 quorum sensing response regulator transcription factor QseB Regulator
  JH656_RS04960 (JH656_04900) ygiW 1012783..1013175 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  JH656_RS04965 (JH656_04905) ygiV 1013228..1013710 (+) 483 WP_100620835.1 GyrI-like domain-containing protein -
  JH656_RS04970 (JH656_04910) mqsR 1013915..1014211 (+) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  JH656_RS04975 (JH656_04915) mqsA 1014213..1014608 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  JH656_RS04980 (JH656_04920) ygiS 1014741..1016348 (+) 1608 WP_001295629.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24742.71 Da        Isoelectric Point: 7.4218

>NTDB_id=456599 JH656_RS04955 WP_139560537.1 1011972..1012631(-) (ciaR) [Escherichia coli strain Ec-RL2-1X]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFRQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=456599 JH656_RS04955 WP_139560537.1 1011972..1012631(-) (ciaR) [Escherichia coli strain Ec-RL2-1X]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTAGACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

36.771

100

0.374
  ciaR Streptococcus pneumoniae D39

36.771

100

0.374
  ciaR Streptococcus pneumoniae R6

36.771

100

0.374
  ciaR Streptococcus pneumoniae TIGR4

36.771

100

0.374
  ciaR Streptococcus mutans UA159

35.874

100

0.365