Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HUW35_RS00180 Genome accession   NZ_CP055157
Coordinates   37642..38826 (-) Length   394 a.a.
NCBI ID   WP_181253733.1    Uniprot ID   -
Organism   Microbulbifer sp. YPW1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 32642..43826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUW35_RS00150 (HUW35_00150) - 33306..33797 (-) 492 WP_181253727.1 BLUF domain-containing protein -
  HUW35_RS00155 (HUW35_00155) - 33902..34816 (-) 915 WP_181253728.1 LysR family transcriptional regulator -
  HUW35_RS00160 (HUW35_00160) - 34965..35444 (+) 480 WP_181253729.1 DoxX family protein -
  HUW35_RS00165 (HUW35_00165) - 35515..36462 (+) 948 WP_181253730.1 glutathione S-transferase family protein -
  HUW35_RS00170 (HUW35_00170) - 36573..36902 (+) 330 WP_181253731.1 hypothetical protein -
  HUW35_RS00175 (HUW35_00175) - 37082..37648 (+) 567 WP_181253732.1 heme-binding protein -
  HUW35_RS00180 (HUW35_00180) pilU 37642..38826 (-) 1185 WP_181253733.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HUW35_RS00185 (HUW35_00185) - 38936..39427 (+) 492 WP_181253734.1 arsinothricin resistance N-acetyltransferase ArsN1 family B -
  HUW35_RS00190 (HUW35_00190) - 39682..40176 (+) 495 WP_255463629.1 NlpC/P60 family protein -
  HUW35_RS00195 (HUW35_00195) - 40189..41481 (-) 1293 WP_219932624.1 carbohydrate porin -
  HUW35_RS00200 (HUW35_00200) - 41639..43480 (-) 1842 WP_255463389.1 sodium:proton antiporter -

Sequence

Protein


Download         Length: 394 a.a.        Molecular weight: 43931.25 Da        Isoelectric Point: 5.6752

>NTDB_id=456430 HUW35_RS00180 WP_181253733.1 37642..38826(-) (pilU) [Microbulbifer sp. YPW1]
MDFTGYLRVLAKNDGSDLYLSTGAPPCAKFQGVLKPLSKEPLGPGEIEKIAMDIMDDEQRDEFKRELEMNLAMTIPNVGR
FRINIFWQRNQVSIVARNIVTEIPKFDDLKLPPILKDVVMAKRGLVLFVGGTGSGKSTSLAALIDHRNTNSGGHIITIED
PVEYVHRHKKSVINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLAISTLHANNANQALDRI
VNFFPEERRPQLLMSLSQNIRAFVSQRLVPTVDGKRCAAVEILLGTQTINELILKGEFHNIKEMMEKSENLGMQTFDGAL
FKLYVEGRISHDEAIKNADSANNLRLRIKMYEEGKKPKAVVEESDEETGAAKTGGGLSLALESIEDEEEDALVY

Nucleotide


Download         Length: 1185 bp        

>NTDB_id=456430 HUW35_RS00180 WP_181253733.1 37642..38826(-) (pilU) [Microbulbifer sp. YPW1]
ATGGACTTTACCGGTTATCTCCGTGTACTCGCCAAAAATGATGGCTCTGACCTCTACTTGAGCACCGGCGCGCCGCCCTG
TGCCAAGTTCCAGGGGGTGCTGAAACCCCTTAGCAAAGAGCCCCTTGGCCCCGGGGAGATCGAGAAGATCGCCATGGATA
TCATGGATGACGAGCAGCGCGATGAGTTCAAGCGCGAGCTGGAAATGAACCTGGCGATGACCATCCCCAATGTGGGGCGC
TTCCGGATCAATATTTTCTGGCAACGCAATCAGGTCTCCATTGTTGCGCGAAATATCGTCACCGAGATTCCCAAGTTTGA
TGACCTCAAGCTGCCGCCTATTCTCAAGGACGTGGTGATGGCCAAGCGCGGCCTGGTGCTGTTTGTGGGCGGTACCGGCT
CGGGCAAGTCCACCTCGCTGGCGGCGCTGATTGACCACCGCAACACCAACAGCGGTGGCCACATCATCACCATTGAAGAC
CCGGTGGAGTATGTGCACCGGCACAAGAAGAGCGTGATCAATCAGCGCGAGGTGGGTGTGGATACCCGCAGCTTCCACGC
GGCGCTGAAGAACACCTTGCGTCAGGCGCCGGACGTGATCTTGATCGGCGAGATCCGCGATCGCGAGACCATGGAGCACG
CCATCGCCTTTGCCGAAACCGGCCACCTGGCAATTTCCACCCTGCATGCCAACAACGCCAACCAGGCCCTCGACCGGATC
GTAAACTTTTTCCCGGAAGAGCGTCGCCCACAACTGCTGATGTCACTGTCGCAGAATATCCGCGCCTTCGTATCCCAGCG
CCTGGTGCCCACGGTGGACGGCAAGCGCTGCGCCGCTGTGGAAATCCTGCTGGGAACACAGACCATCAACGAGCTGATCC
TGAAAGGCGAGTTCCACAACATCAAGGAAATGATGGAAAAGTCAGAAAACCTCGGTATGCAGACGTTTGACGGTGCGCTG
TTCAAACTGTACGTGGAAGGCCGTATCAGCCACGACGAGGCGATCAAGAACGCGGACTCTGCCAATAACCTGCGCCTGCG
GATCAAAATGTATGAAGAGGGCAAGAAGCCCAAGGCGGTGGTCGAGGAATCCGACGAGGAAACTGGAGCGGCCAAGACCG
GTGGTGGCTTGTCGCTCGCGCTGGAGTCGATCGAGGACGAAGAGGAAGATGCGCTGGTTTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.085

89.848

0.495
  pilU Acinetobacter baylyi ADP1

53.76

91.117

0.49
  pilU Vibrio cholerae strain A1552

49.143

88.832

0.437
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.773

86.041

0.368
  pilT Legionella pneumophila strain Lp02

42.814

84.772

0.363
  pilT Legionella pneumophila strain ERS1305867

42.814

84.772

0.363