Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   FOC85_RS18585 Genome accession   NZ_CP054568
Coordinates   3569501..3573016 (+) Length   1171 a.a.
NCBI ID   WP_000058569.1    Uniprot ID   A0RAX6
Organism   Bacillus thuringiensis strain FDAARGOS_791     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3564501..3578016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOC85_RS18550 (FOC85_18555) - 3564820..3565608 (-) 789 WP_000122656.1 phosphotransferase -
  FOC85_RS18555 (FOC85_18560) cspA 3565960..3566163 (+) 204 WP_000301519.1 RNA chaperone/antiterminator CspA -
  FOC85_RS18560 (FOC85_18565) - 3566351..3566563 (-) 213 WP_000412671.1 hypothetical protein -
  FOC85_RS18565 (FOC85_18570) - 3566849..3567034 (-) 186 WP_000391382.1 hypothetical protein -
  FOC85_RS18570 (FOC85_18575) - 3567316..3567897 (+) 582 WP_000428957.1 competence protein ComK -
  FOC85_RS18575 (FOC85_18580) - 3568171..3568764 (+) 594 WP_000347516.1 TVP38/TMEM64 family protein -
  FOC85_RS18580 (FOC85_18585) lepB 3568821..3569384 (+) 564 WP_000751894.1 signal peptidase I -
  FOC85_RS18585 (FOC85_18590) addB 3569501..3573016 (+) 3516 WP_000058569.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  FOC85_RS18590 (FOC85_18595) addA 3573013..3576738 (+) 3726 WP_000572292.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  FOC85_RS18595 (FOC85_18600) - 3576751..3577038 (+) 288 WP_000718623.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  FOC85_RS18600 (FOC85_18605) gerPF 3577076..3577291 (-) 216 WP_001141566.1 spore germination protein GerPF -
  FOC85_RS18605 (FOC85_18610) - 3577334..3577720 (-) 387 WP_000902341.1 spore germination protein GerPE -
  FOC85_RS18610 (FOC85_18615) gerPD 3577736..3577930 (-) 195 WP_001052802.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134312.48 Da        Isoelectric Point: 5.3727

>NTDB_id=453440 FOC85_RS18585 WP_000058569.1 3569501..3573016(+) (addB) [Bacillus thuringiensis strain FDAARGOS_791]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVENLPQSEYVKGAEVYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEMHYEARPNEKFHGEASVTIHTAANL
RAEVEGVAREIRRLVAEENYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSAAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPNVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTAVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSTVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKSLDLTEVYYGLALQMLTYLDVVTSNAQTWMKKGHAASPAGVLYFHIHNPIVEVKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFKNIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=453440 FOC85_RS18585 WP_000058569.1 3569501..3573016(+) (addB) [Bacillus thuringiensis strain FDAARGOS_791]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAATTAAA
ACAGCGTCCAAGAGGGGAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGGCA
GTGAGGATGTTAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTATTGCAAGAAGTTGGT
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTACATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTGGGCAGTATGATTGCGGAGTTTAAACGATATA
ATGTGACGCCATCAAACGTATATGAAATGTGGCAACAATTAGATGCCCATAGTAGCAGTGCAGAACAAAAGCTACTGGCG
AATAAAGTGTATGATTTACAACTACTATATGATGATTTTGAGCGTGCTTTAATCGGAAAATATTTAGATTCAGAAGACTA
TCTGCAATTGCTAGTCGAAAATCTTCCTCAATCTGAATATGTAAAGGGAGCTGAAGTTTATATAGATGGATTTCACTCAT
TTTCACCGCAAGAGTTAGAAATTGTAAGACAGCTTATGATTTGCGGAGCGAGAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAATTAGATTTATTTTATGAAACGACATTAACGTATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACGATTCCACTTATGGAACAGCCGCGTTTTCATTCTCCGGCATTAGCGCATT
TAGAAATGCATTACGAAGCGCGTCCAAATGAAAAGTTTCACGGTGAAGCAAGTGTAACCATTCATACAGCAGCTAATTTA
CGAGCGGAAGTAGAAGGCGTTGCTCGTGAAATTCGTAGACTTGTGGCTGAAGAAAACTATCGTTACCGAGATATTGCGGT
TCTTCTTCGTAACGGGGAAAGTTATTACGATGTAATGCGGACACTATTTACAGATTATAATATCCCGCACTTCATCGATG
AAAAACGCCCGATGTCACATCATCCGTTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTCTTTCGCTGCGTGAAAACAGAGCTTTTATATCCACTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTATTGCTTAGCATACGGTGTACAAGGGAAGAGATGGACTTCTGAGGATCCGTGGATGTATCGTCGCT
ATCGTTCTCTTGACGATACGAACGGGATGATCACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATCGATTGCGTGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGACTGAAGCGCGCGGGAACAGTTATGCAAATGTGCGAAGCTGTTTA
CTTATTTTTAGAAGAGCTGGACGTTCCAAAAAAATTAGAAGCATTACGTATTCGTGCAGAAGAGAGTGGGGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTTATGAGTCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGAGGCGCTTCAATTCGCAAACATTCCGCCGTCATTAGA
CCAAGTGTTGATTGCTAATATTGATCGTTCAAGATTATCAAATGTGAAAGCAACATTTGTTATTGGCGTGAATGAAGGTG
TCATTCCAGCAGCACCGATGGATGAAGGTATGCTTTCTGATGAGGAAAGAGATGTTTTGAGCGCTGCAGGTATTGAACTC
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
TATTTCATGCCCGCTTGCAGATGAGGAAGGGAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTAATGTGAAAGATACATTTATTACGAATGACGTAAATGATTTATCACGTTCGGAACAAATTTCATACGTAGCAACGCCG
GAAGTAACACTGTCATATGTTATGCAGCAACTTCAAACGTGGAAGCGATATGGATTTGAAGGGAATTTAGACTTTTGGTG
GGATGTATATAATTTCTACGTAACTTCGGATGAATGGAAGCAAAAAAGTAGCCGCGTATTATCAAGTTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGCTGTAAGTAGAGATTTATACGGAGACAAAATAAAAGGAAGCGTTTCTCGTATGGAA
CTATTTAATCGTTGTGCGTACGCTCATTTCGCACAGCACGGTTTATCGTTAAGAGAGCGTGATATTTTTAAACTTGATGC
GCCAGATATCGGGGAGCTTTTCCATGCAGCGCTGAAGAGAATTGCAGACAGGCTATTACGTGAAAATCGTACTTGGGCAG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTACTGTAGTAATAGAAGAGATTGCACCGTTATTACAAAGGCAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCCATTATTCTACGTGAACA
TGCGAAGTCGAGCGGTTTCGTACCAGTTGATTTAGAAGTGCCATTTGGTATGGGCGGTACAGGATCACTTCCGCCGATGG
AATTCTCGTTACCAAATGGTGTAAAGATGGAAGTAGTCGGCCGTATTGACCGCGTTGATAAGGCGGAAGATGAAAACGGA
ACATTCCTCCGTATTATTGACTATAAATCAAGCTCAAAATCGTTAGACTTAACGGAAGTGTATTACGGATTGGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACAGACGTGGATGAAAAAAGGCCACGCAGCATCACCAGCTGGTG
TACTGTACTTCCACATTCATAACCCAATTGTTGAGGTGAAAGGTGACGCATCTGAAGCAGAAATTGAAAAGGAAATTTTA
AAGAAATTCAAAATGAAAGGGCTCGTACTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
TTCAGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGTTTTAGTGCGCGTTCAAGTATTGCCAGTGAACAAGAGTTTA
ACGTCCTGCAAAAATACGTACATCATACGTTTAAAAATATCGGAAAAGACATTACAGAGGGTGTTATTGATATTGCTCCA
TACAAAAAGGGGAATAAAGCAGCGTGTACGTTCTGTAACTTCAAATCAGTTTGTCAGTTCGATGAATCACTTGAAGATAA
TCAATTCCGTACGCTAAAAGATATGAAAGATAGTGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(304-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0RAX6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.147

100

0.492