NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	HTY60_RS13060	Genome accession	NZ_CP054415
Coordinates	2527735..2528349 (-)	Length	204 a.a.
NCBI ID	WP_013352942.1	Uniprot ID	A0A411A874
Organism	Bacillus amyloliquefaciens strain 205
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2522735..2533349

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HTY60_RS13030 (HTY60_13030)	rpsT	2523035..2523301 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
HTY60_RS13035 (HTY60_13035)	holA	2523318..2524359 (-)	1042	Protein_2568	DNA polymerase III subunit delta	-
HTY60_RS13040 (HTY60_13040)	-	2524399..2524553 (-)	155	Protein_2569	hypothetical protein	-
HTY60_RS13045 (HTY60_13045)	-	2524594..2524728 (+)	135	WP_003152870.1	YqzM family protein	-
HTY60_RS13050 (HTY60_13050)	comEC	2524747..2527098 (-)	2352	WP_041481655.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
HTY60_RS13055 (HTY60_13055)	-	2527099..2527668 (-)	570	WP_003152868.1	ComE operon protein 2	-
HTY60_RS13060 (HTY60_13060)	comEA	2527735..2528349 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
HTY60_RS13065 (HTY60_13065)	comER	2528408..2529229 (+)	822	WP_013352943.1	late competence protein ComER	-
HTY60_RS13070 (HTY60_13070)	-	2529298..2530035 (-)	738	WP_013352944.1	class I SAM-dependent methyltransferase	-
HTY60_RS13075 (HTY60_13075)	rsfS	2530032..2530388 (-)	357	WP_003152864.1	ribosome silencing factor	-
HTY60_RS13080 (HTY60_13080)	yqeK	2530406..2530966 (-)	561	WP_013352945.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
HTY60_RS13085 (HTY60_13085)	-	2530956..2531525 (-)	570	WP_013352946.1	nicotinate-nucleotide adenylyltransferase	-
HTY60_RS13090 (HTY60_13090)	yhbY	2531536..2531826 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
HTY60_RS13095 (HTY60_13095)	aroE	2531820..2532662 (-)	843	WP_013352947.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22190.27 Da Isoelectric Point: 5.4841

                                >NTDB_id=452324 HTY60_RS13060 WP_013352942.1 2527735..2528349(-) (comEA) [Bacillus amyloliquefaciens strain 205]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=452324 HTY60_RS13060 WP_013352942.1 2527735..2528349(-) (comEA) [Bacillus amyloliquefaciens strain 205]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCCT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG

CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A411A874

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0452324