Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   HR084_RS12850 Genome accession   NZ_CP054177
Coordinates   2531734..2532351 (-) Length   205 a.a.
NCBI ID   WP_014480312.1    Uniprot ID   -
Organism   Bacillus subtilis strain JCL16     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2526734..2537351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HR084_RS12820 (HR084_12820) rpsT 2527036..2527302 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  HR084_RS12825 (HR084_12825) holA 2527317..2528360 (-) 1044 WP_014480310.1 DNA polymerase III subunit delta -
  HR084_RS12830 (HR084_12830) - 2528400..2528549 (-) 150 WP_003229985.1 hypothetical protein -
  HR084_RS12835 (HR084_12835) yqzM 2528590..2528724 (+) 135 WP_003229983.1 YqzM family protein -
  HR084_RS12840 (HR084_12840) comEC 2528764..2531094 (-) 2331 WP_033881047.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  HR084_RS12845 (HR084_12845) comEB 2531098..2531667 (-) 570 WP_003229978.1 ComE operon protein 2 -
  HR084_RS12850 (HR084_12850) comEA 2531734..2532351 (-) 618 WP_014480312.1 competence protein ComEA Machinery gene
  HR084_RS12855 (HR084_12855) comER 2532435..2533256 (+) 822 WP_014480313.1 late competence protein ComER -
  HR084_RS12860 (HR084_12860) yqeM 2533322..2534065 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  HR084_RS12865 (HR084_12865) rsfS 2534062..2534418 (-) 357 WP_041333759.1 ribosome silencing factor -
  HR084_RS12870 (HR084_12870) yqeK 2534436..2534996 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  HR084_RS12875 (HR084_12875) nadD 2534986..2535555 (-) 570 WP_041333756.1 nicotinate-nucleotide adenylyltransferase -
  HR084_RS12880 (HR084_12880) yhbY 2535567..2535857 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  HR084_RS12885 (HR084_12885) aroE 2535851..2536693 (-) 843 WP_173614193.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21781.51 Da        Isoelectric Point: 4.7220

>NTDB_id=451341 HR084_RS12850 WP_014480312.1 2531734..2532351(-) (comEA) [Bacillus subtilis strain JCL16]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=451341 HR084_RS12850 WP_014480312.1 2531734..2532351(-) (comEA) [Bacillus subtilis strain JCL16]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.512

100

0.995
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39