Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexA   Type   Machinery gene
Locus tag   ST4147_RS00390 Genome accession   NZ_CP065504
Coordinates   64239..66797 (+) Length   852 a.a.
NCBI ID   WP_011226740.1    Uniprot ID   Q5M1Z0
Organism   Streptococcus thermophilus strain 4147     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 59239..71797
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ST4147_RS00355 (ST4147_00355) - 59856..60038 (+) 183 WP_014727424.1 hypothetical protein -
  ST4147_RS00360 (ST4147_00360) - 60838..61308 (+) 471 WP_347229952.1 hypothetical protein -
  ST4147_RS00365 (ST4147_00365) - 61372..61908 (+) 537 WP_347229953.1 hypothetical protein -
  ST4147_RS00370 (ST4147_00370) - 62430..62639 (+) 210 WP_347229954.1 helix-turn-helix transcriptional regulator -
  ST4147_RS00375 (ST4147_00375) prx 62963..63148 (+) 186 WP_347229955.1 Paratox Regulator
  ST4147_RS00380 (ST4147_00380) argR 63459..63887 (+) 429 WP_024704313.1 arginine repressor -
  ST4147_RS00385 (ST4147_00385) - 63887..64246 (+) 360 WP_011225277.1 YlbF family regulator -
  ST4147_RS00390 (ST4147_00390) hexA 64239..66797 (+) 2559 WP_011226740.1 DNA mismatch repair protein MutS Machinery gene
  ST4147_RS00395 (ST4147_00395) - 66831..67286 (-) 456 Protein_57 DUF3021 domain-containing protein -
  ST4147_RS00400 (ST4147_00400) - 67283..67674 (-) 392 Protein_58 LytTR family DNA-binding domain-containing protein -
  ST4147_RS00405 (ST4147_00405) - 67898..68203 (+) 306 WP_002949050.1 hypothetical protein -
  ST4147_RS00410 (ST4147_00410) hexB 68255..70198 (+) 1944 WP_011225282.1 DNA mismatch repair endonuclease MutL Machinery gene
  ST4147_RS00415 (ST4147_00415) - 70328..70549 (+) 222 WP_041826940.1 hypothetical protein -
  ST4147_RS00420 (ST4147_00420) ruvA 70701..71291 (+) 591 WP_002949053.1 Holliday junction branch migration protein RuvA Machinery gene

Sequence

Protein


Download         Length: 852 a.a.        Molecular weight: 95437.71 Da        Isoelectric Point: 4.7412

>NTDB_id=449624 ST4147_RS00390 WP_011226740.1 64239..66797(+) (hexA) [Streptococcus thermophilus strain 4147]
MAKEKISPGMQQYLDIKKNYPDAFLLFRMGDFYELFYDDAVKAAQILEISLTSRNKNADNPIPMAGVPYHSAQAYIDVLV
EMGYKVAIAEQMEDPKQAVGVVKREVVQVITPGTVVDSSKPDSANNFLVAIDKVGSRFGLSYMDVSTGEFFATELDDFSS
VCSEIQNLKAREVVVGYDLLETDEQVLVNQLNLLLSKETEGYDDVHLIGNSLTDLESSVASKLLQYVHRTQMRELSHLQK
AQHYEIKDYLQMSYATKSSLDLLENARTGKKHGSLFWLLDKTKTAMGMRLLRTWIDRPLVNQASIIERQNIIQVFLDNFF
ERSDLAESLKGVYDIERLASRVSFGKANPKDLIQLGHTLAQVPVIKAILESFNDDALSGLLQELDALPELESLIRSAIDP
DAPATITEGGIIRDGFDETLDKYRKVMSEGTSWIADIEAKEREASGITTLKIDYNRKDGYYFHVTNSNLSLVPDHFFRKA
TLKNSERFGTAELAKIEGEMLEAREKSSTLEYDIFMRVREQVERYIDRLQSLAKAIATVDVLQSLAVTAETNHYVRPVFN
DEHRIAIDRGRHAVVEKVMGVQEYIPNTITFDSQTNIQLITGPNMSGKSTYMRQLALSVVMAQMGAYVPADSVDLPVFDA
IYTRIGAADDLISGQSTFMVEMMEANQAIKRATPNSLIIFDELGRGTATYDGMALAQSIIEFIHDKVGAKTMFATHYHEL
TALSNSLTHLVNVHVATLEKDGEVTFLHKIVDGPADKSYGIHVAKIAGLPTDLLNRADTILTQLEGETVVIQPQEKVLSQ
EKPAIETHVNEQISLFDDFTENPVLQELRDLDIYNMTPMQVMMAVADLKQKL

Nucleotide


Download         Length: 2559 bp        

>NTDB_id=449624 ST4147_RS00390 WP_011226740.1 64239..66797(+) (hexA) [Streptococcus thermophilus strain 4147]
ATGGCTAAGGAAAAAATATCTCCAGGAATGCAGCAGTATTTGGATATTAAGAAAAATTATCCAGATGCTTTTTTGCTGTT
TCGGATGGGGGATTTTTACGAATTATTTTACGATGATGCTGTTAAGGCTGCCCAGATTTTGGAAATTAGCCTAACTAGCC
GTAATAAGAATGCAGATAATCCCATTCCTATGGCAGGTGTGCCTTATCATTCAGCTCAAGCATACATTGATGTTTTGGTT
GAGATGGGCTACAAGGTAGCCATTGCTGAGCAGATGGAGGATCCTAAGCAGGCTGTCGGTGTGGTAAAGCGTGAGGTTGT
TCAGGTTATCACACCAGGTACAGTGGTTGACAGCTCTAAACCTGATAGTGCCAACAATTTCTTGGTGGCCATTGACAAGG
TTGGAAGTCGTTTTGGTCTTTCCTATATGGATGTGTCAACGGGTGAATTTTTTGCGACAGAGTTGGATGATTTTAGTTCA
GTTTGTAGTGAAATTCAGAACCTTAAGGCACGTGAAGTAGTGGTTGGATATGATCTACTTGAAACTGACGAACAAGTTTT
AGTGAACCAACTTAACCTACTTCTCTCCAAGGAAACAGAAGGTTACGATGATGTTCACTTGATTGGTAATAGCTTGACAG
ATTTGGAAAGCAGTGTGGCAAGTAAACTTCTCCAGTATGTTCATCGCACTCAGATGCGTGAGCTCAGTCATTTACAAAAA
GCTCAGCACTATGAGATAAAGGATTACTTGCAGATGTCCTATGCGACCAAGTCAAGCTTGGATTTACTGGAAAATGCTAG
AACGGGCAAGAAACACGGTTCTCTTTTCTGGCTCTTGGATAAAACCAAGACAGCCATGGGAATGCGTCTCTTGCGAACTT
GGATTGATCGTCCTTTAGTGAATCAAGCCAGCATCATAGAGCGTCAGAATATTATCCAAGTTTTCTTGGACAATTTCTTT
GAGCGTAGTGACTTGGCCGAGAGTCTAAAAGGTGTTTACGATATTGAGCGTCTGGCCAGTCGTGTTTCCTTTGGCAAGGC
TAATCCTAAAGATTTAATTCAGCTAGGTCATACCTTAGCTCAGGTTCCGGTCATTAAGGCAATCTTGGAGTCCTTTAATG
ACGATGCCTTGTCAGGTCTTTTACAAGAGTTAGATGCTTTGCCAGAATTAGAGAGTTTGATTCGTTCGGCTATTGATCCT
GATGCTCCAGCAACAATTACAGAAGGTGGTATTATTCGTGATGGTTTTGATGAGACCTTGGACAAGTATCGCAAAGTGAT
GAGCGAGGGGACGTCATGGATTGCGGATATCGAAGCAAAAGAACGCGAAGCATCTGGTATTACAACACTAAAAATTGATT
ATAACCGTAAAGATGGTTATTACTTCCATGTGACCAACTCTAACCTTAGTTTGGTTCCTGACCATTTCTTCCGTAAGGCG
ACACTGAAAAATTCGGAGCGTTTTGGAACTGCTGAGTTGGCTAAGATTGAAGGTGAAATGCTAGAAGCACGTGAAAAATC
GTCAACCCTAGAGTATGATATTTTCATGCGTGTCCGTGAGCAGGTTGAACGCTATATCGACCGTTTGCAGTCTTTGGCTA
AGGCCATTGCAACAGTCGATGTGCTTCAGAGTTTGGCTGTTACAGCGGAGACAAATCATTATGTTCGTCCTGTTTTTAAT
GATGAGCATCGTATCGCTATTGATCGGGGCCGTCATGCGGTGGTTGAGAAGGTTATGGGCGTTCAGGAATACATTCCAAA
TACGATTACCTTTGACAGTCAGACCAATATTCAGCTCATCACAGGACCAAATATGAGTGGTAAATCTACCTATATGCGGC
AGTTAGCCTTGTCAGTAGTTATGGCTCAAATGGGGGCTTACGTACCAGCAGATAGTGTTGATTTACCTGTTTTTGACGCC
ATCTATACACGTATAGGTGCCGCAGATGACCTGATTTCAGGTCAGTCGACCTTCATGGTTGAGATGATGGAGGCTAATCA
GGCCATTAAGCGAGCGACACCTAATTCCTTGATTATTTTTGATGAGTTGGGGCGTGGGACAGCCACTTATGATGGTATGG
CTTTGGCCCAGTCTATTATTGAGTTTATTCATGACAAGGTTGGGGCTAAGACCATGTTTGCTACCCATTATCATGAGTTG
ACTGCGCTGTCAAACAGTTTGACACACCTTGTCAACGTCCATGTTGCGACACTTGAAAAGGATGGCGAAGTGACTTTCCT
CCACAAAATTGTCGATGGTCCAGCCGATAAATCTTATGGTATTCATGTAGCTAAAATTGCAGGTTTACCAACAGATTTAT
TGAACCGTGCTGACACCATATTGACACAATTAGAAGGAGAGACAGTTGTAATCCAACCTCAGGAAAAGGTATTGTCTCAA
GAAAAGCCTGCCATTGAAACGCATGTCAACGAGCAAATCTCTCTCTTTGATGATTTTACTGAAAATCCGGTTCTTCAAGA
GTTGCGTGATTTGGACATTTACAACATGACACCGATGCAAGTCATGATGGCTGTAGCAGATTTAAAACAGAAACTATAA

Domains

Predicted by InterProScan.

MutS domain I (MutS_I)

(8-118)

MutS domain II (MutS_II)

(126-239)

MutS family domain IV (MutS_IV)

(415-505)

MutS domain V (MutS_V)

(599-785)

MutS domain III (MutS_III)

(256-546)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5M1Z0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexA Streptococcus pneumoniae R6

73.826

100

0.738
  mutS Pseudomonas stutzeri strain ATCC 17587

35.788

100

0.365