Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexB   Type   Machinery gene
Locus tag   ST4147_RS00410 Genome accession   NZ_CP065504
Coordinates   68255..70198 (+) Length   647 a.a.
NCBI ID   WP_011225282.1    Uniprot ID   Q5M6H7
Organism   Streptococcus thermophilus strain 4147     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 63255..75198
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ST4147_RS00380 (ST4147_00380) argR 63459..63887 (+) 429 WP_024704313.1 arginine repressor -
  ST4147_RS00385 (ST4147_00385) - 63887..64246 (+) 360 WP_011225277.1 YlbF family regulator -
  ST4147_RS00390 (ST4147_00390) hexA 64239..66797 (+) 2559 WP_011226740.1 DNA mismatch repair protein MutS Machinery gene
  ST4147_RS00395 (ST4147_00395) - 66831..67286 (-) 456 Protein_57 DUF3021 domain-containing protein -
  ST4147_RS00400 (ST4147_00400) - 67283..67674 (-) 392 Protein_58 LytTR family DNA-binding domain-containing protein -
  ST4147_RS00405 (ST4147_00405) - 67898..68203 (+) 306 WP_002949050.1 hypothetical protein -
  ST4147_RS00410 (ST4147_00410) hexB 68255..70198 (+) 1944 WP_011225282.1 DNA mismatch repair endonuclease MutL Machinery gene
  ST4147_RS00415 (ST4147_00415) - 70328..70549 (+) 222 WP_041826940.1 hypothetical protein -
  ST4147_RS00420 (ST4147_00420) ruvA 70701..71291 (+) 591 WP_002949053.1 Holliday junction branch migration protein RuvA Machinery gene
  ST4147_RS00425 (ST4147_00425) - 71298..71853 (+) 556 Protein_63 DNA-3-methyladenine glycosylase I -
  ST4147_RS00430 (ST4147_00430) cinA 71949..73220 (+) 1272 WP_011226745.1 competence/damage-inducible protein A Machinery gene
  ST4147_RS00435 (ST4147_00435) recA 73256..74410 (+) 1155 WP_011226746.1 recombinase RecA Machinery gene
  ST4147_RS00440 (ST4147_00440) spx 74587..74985 (+) 399 WP_002949058.1 transcriptional regulator Spx -

Sequence

Protein


Download         Length: 647 a.a.        Molecular weight: 72504.77 Da        Isoelectric Point: 5.9934

>NTDB_id=449625 ST4147_RS00410 WP_011225282.1 68255..70198(+) (hexB) [Streptococcus thermophilus strain 4147]
MPKIIELPEVLANQIAAGEVVERPASVVKELVENAIDAGSTQITIEVEESGLSKIQITDNGEGMAQADVAMSLRRHATSK
IKNQGDLFRIRTLGFRGEALPSIASISHLTIVTAADGEVYGTKLVAKGGEIESQDPISTPVGTKITVENLFYNTPARLKY
MKSLQAELAHIVDVVNRLSLAHPEVAFTLLNDGRQLTQTSGTGDLRQAIAGIYGLTTAKKMVEISNSDLDFEVSGYVSLP
ELTRANRNYITILINGRYIKNFLLNRAIFDGYGSKLMVGRFPIAVIDIQIDPYLADVNVHPTKQEVRISKEKELMALIKS
AIAQSLREQDLIPDALENLAKSSTRGATRSVQTSLPLKQTNLYYDSSRNDFFVTPETVQEDIKPLVSKSESSVSLVANKQ
QPTVKQAKRSADDSDSEHGKLDYKNKSKLKRMLENLTNEETSTFPELEFFGQMHGTYLFAQGQGGLYIIDQHAAQERVKY
EYYREKIGVVDSSLQQLLVPYLFEFSGSDYISLQEKMPLLNQVCIYLEPYGNNTFILREHPIWMKEEEIESAVYEMCDML
LLTNEVSVKTYRAELAIMMSCKRSIKANHALDDYSARDLLVQLAQCKNPYNCPHGRPVLVNFTKSDMEKMFRRIQENHTS
LRDLGKY

Nucleotide


Download         Length: 1944 bp        

>NTDB_id=449625 ST4147_RS00410 WP_011225282.1 68255..70198(+) (hexB) [Streptococcus thermophilus strain 4147]
ATGCCAAAAATTATCGAATTGCCTGAAGTTCTAGCCAATCAGATTGCTGCTGGTGAGGTCGTAGAGCGACCAGCTAGTGT
AGTTAAAGAGCTGGTGGAAAATGCTATTGATGCAGGCAGCACCCAGATTACTATTGAAGTTGAAGAATCAGGCCTCTCAA
AAATTCAAATCACAGACAATGGCGAAGGAATGGCTCAAGCTGATGTAGCTATGAGTTTGCGCCGTCATGCAACGAGTAAA
ATCAAAAATCAAGGAGATCTCTTTCGTATTCGAACACTTGGTTTTCGAGGTGAAGCCCTACCCTCTATAGCTTCTATTAG
TCACCTTACCATTGTGACAGCAGCGGATGGAGAAGTCTATGGGACCAAACTTGTTGCTAAAGGTGGAGAAATTGAGAGTC
AAGATCCTATTTCAACCCCAGTGGGAACAAAGATTACAGTTGAAAACTTGTTTTATAACACCCCAGCTCGTCTCAAGTAT
ATGAAGAGTTTACAGGCTGAGTTGGCTCATATTGTCGATGTTGTCAACCGTTTGAGTTTAGCCCACCCCGAAGTAGCCTT
CACCTTGCTTAATGATGGTCGTCAGTTGACACAGACATCAGGTACTGGAGACCTTCGCCAAGCTATTGCAGGGATTTATG
GCTTAACAACAGCAAAAAAAATGGTGGAAATTTCAAATTCCGACCTTGATTTTGAAGTTTCTGGTTATGTCAGTCTCCCT
GAATTAACGCGTGCTAACCGTAACTACATCACGATTCTCATTAACGGACGCTATATAAAAAACTTCCTCCTTAACCGTGC
TATTTTTGATGGTTATGGTTCTAAACTTATGGTGGGACGCTTCCCAATTGCAGTTATTGATATTCAGATTGATCCTTATT
TGGCAGATGTCAATGTTCACCCAACAAAGCAAGAAGTACGTATCTCTAAAGAAAAGGAGCTCATGGCCCTTATCAAATCT
GCCATTGCTCAAAGCCTTAGAGAGCAGGATTTGATTCCTGATGCCCTTGAAAATCTAGCAAAGTCAAGTACTAGAGGTGC
AACTAGGTCAGTTCAGACGAGTCTCCCACTCAAACAAACCAATCTTTACTATGACAGTAGCCGTAATGATTTCTTTGTCA
CGCCAGAGACAGTTCAAGAGGATATCAAACCTCTTGTATCAAAGTCAGAGTCTTCAGTTTCATTGGTAGCAAACAAACAG
CAACCTACTGTTAAACAGGCAAAACGTTCAGCAGATGATAGTGATAGCGAACACGGAAAACTTGACTATAAAAATAAATC
GAAATTGAAGCGCATGCTTGAAAATCTGACCAATGAGGAAACATCAACCTTTCCTGAATTGGAGTTCTTTGGTCAAATGC
ATGGAACCTACCTCTTTGCCCAAGGACAAGGTGGCCTTTACATCATTGACCAGCATGCTGCCCAAGAACGTGTCAAGTAT
GAGTACTATCGCGAAAAGATAGGTGTGGTTGATAGTAGTTTACAACAACTTTTGGTTCCCTATCTTTTTGAATTTTCAGG
TTCAGATTACATTAGTTTACAAGAAAAAATGCCTCTTCTTAACCAAGTTTGCATCTATTTGGAGCCTTATGGGAATAATA
CCTTCATTCTTCGAGAGCACCCTATCTGGATGAAGGAAGAAGAAATCGAATCTGCAGTCTATGAGATGTGTGATATGCTT
CTTTTGACGAACGAAGTATCCGTCAAGACCTATCGTGCTGAGTTAGCCATCATGATGAGTTGTAAACGTTCAATAAAGGC
CAATCATGCTCTTGATGATTACTCTGCGCGTGACCTTCTAGTACAGCTTGCACAGTGTAAAAATCCCTATAATTGTCCTC
ACGGCCGTCCAGTACTTGTCAACTTTACCAAGTCTGATATGGAAAAAATGTTCCGTCGTATTCAAGAAAACCATACCAGC
CTACGTGATTTAGGAAAGTATTAA

Domains

Predicted by InterProScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c_3)

(25-124)

MutL C terminal dimerisation domain (MutL_C)

(448-591)

DNA mismatch repair protein, C-terminal domain (DNA_mis_repair)

(209-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5M6H7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexB Streptococcus pneumoniae R6

69.419

100

0.702