Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGE   Type   Machinery gene
Locus tag   STMM20_RS00920 Genome accession   NZ_CP065485
Coordinates   177992..178285 (+) Length   97 a.a.
NCBI ID   WP_011226625.1    Uniprot ID   Q5M2G2
Organism   Streptococcus thermophilus strain MM20     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 172992..183285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STMM20_RS00895 (STMM20_00935) - 174943..175305 (+) 363 WP_023909982.1 DUF1033 family protein -
  STMM20_RS00900 (STMM20_00940) comGA 175386..176327 (+) 942 WP_347103581.1 competence type IV pilus ATPase ComGA Machinery gene
  STMM20_RS00905 (STMM20_00945) comGB 176209..177309 (+) 1101 WP_120764773.1 competence type IV pilus assembly protein ComGB Machinery gene
  STMM20_RS00910 (STMM20_00950) comGC 177306..177632 (+) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  STMM20_RS00915 (STMM20_00955) comGD 177592..178020 (+) 429 WP_257608452.1 competence type IV pilus minor pilin ComGD Machinery gene
  STMM20_RS00920 (STMM20_00960) comGE 177992..178285 (+) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE Machinery gene
  STMM20_RS00925 (STMM20_00965) comGF 178269..178706 (+) 438 WP_014608742.1 competence type IV pilus minor pilin ComGF Machinery gene
  STMM20_RS00930 (STMM20_00970) comGG 178684..179001 (+) 318 WP_002952035.1 competence type IV pilus minor pilin ComGG Machinery gene
  STMM20_RS00935 (STMM20_00975) comGH 179046..180002 (+) 957 WP_347103582.1 class I SAM-dependent methyltransferase Machinery gene
  STMM20_RS00940 (STMM20_00980) - 180058..181251 (+) 1194 WP_014608740.1 acetate kinase -
  STMM20_RS00945 (STMM20_00985) - 181500..181697 (+) 198 WP_011681682.1 helix-turn-helix transcriptional regulator -
  STMM20_RS00950 - 181709..181984 (+) 276 WP_011681681.1 hypothetical protein -
  STMM20_RS00955 - 182028..182366 (+) 339 WP_224103197.1 CPBP family intramembrane glutamic endopeptidase -
  STMM20_RS00960 (STMM20_00995) - 182440..182883 (+) 444 WP_011681680.1 hypothetical protein -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10844.74 Da        Isoelectric Point: 10.4561

>NTDB_id=448179 STMM20_RS00920 WP_011226625.1 177992..178285(+) (comGE) [Streptococcus thermophilus strain MM20]
MVNIKRRSLRGYILIESLVAMAVLVLVSSLILEQINTNRRLMADNLHQQEVLSVATMAVQTKQDQLTLNGIAVTVKRSQQ
GITVYESGKEIIHVSKQ

Nucleotide


Download         Length: 294 bp        

>NTDB_id=448179 STMM20_RS00920 WP_011226625.1 177992..178285(+) (comGE) [Streptococcus thermophilus strain MM20]
GTGGTAAATATAAAAAGACGATCTCTTAGAGGTTATATTCTTATTGAAAGTCTGGTAGCTATGGCTGTTTTAGTACTTGT
CAGCAGTCTGATATTGGAACAAATTAATACCAACCGCAGACTAATGGCTGATAATCTCCATCAACAAGAGGTCTTGAGTG
TGGCAACAATGGCAGTTCAGACCAAGCAAGACCAGTTAACGCTGAATGGCATAGCGGTGACAGTTAAACGTAGCCAGCAA
GGTATCACGGTTTATGAATCGGGAAAGGAGATTATTCATGTATCTAAGCAGTAA

Domains

Predicted by InterProScan.

Type II secretory pathway pseudopilin (ComGE)

(13-94)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5M2G2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGE Streptococcus mutans UA140

43.299

100

0.433
  comGE Streptococcus mutans UA159

43.299

100

0.433
  comGE Lactococcus lactis subsp. cremoris KW2

36.082

100

0.361