Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CHHT_RS03025 Genome accession   NZ_CP053827
Coordinates   563826..564893 (+) Length   355 a.a.
NCBI ID   WP_034962660.1    Uniprot ID   A0A855N5J6
Organism   Campylobacter hyointestinalis subsp. hyointestinalis strain CCUG 14169     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 558826..569893
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHHT_RS02990 (CHHT_0595) brnQ 558907..560208 (-) 1302 WP_034962653.1 branched-chain amino acid transport system II carrier protein -
  CHHT_RS02995 (CHHT_0596) hisG 560217..560828 (-) 612 WP_034962654.1 ATP phosphoribosyltransferase -
  CHHT_RS03000 (CHHT_0597) - 560816..561448 (-) 633 WP_034962655.1 type III pantothenate kinase -
  CHHT_RS03005 (CHHT_0598) - 561435..561761 (-) 327 WP_034962656.1 hypothetical protein -
  CHHT_RS03010 (CHHT_0599) - 561763..562761 (-) 999 WP_034962657.1 L-seryl-tRNA selenium transferase -
  CHHT_RS03015 (CHHT_0600) - 562766..563326 (-) 561 WP_034962658.1 hypothetical protein -
  CHHT_RS03020 (CHHT_0601) gatC 563534..563821 (+) 288 WP_034962659.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  CHHT_RS03025 (CHHT_0602) pilT 563826..564893 (+) 1068 WP_034962660.1 type IV pilus twitching motility protein PilT Machinery gene
  CHHT_RS03030 (CHHT_0603) - 564893..565462 (+) 570 WP_230937449.1 CvpA family protein -
  CHHT_RS03035 (CHHT_0604) - 565465..565935 (+) 471 WP_034962843.1 Fur family transcriptional regulator -
  CHHT_RS03040 (CHHT_0605) lysS 565950..567455 (+) 1506 WP_234945122.1 lysine--tRNA ligase -
  CHHT_RS03045 (CHHT_0606) - 567457..568701 (+) 1245 WP_034962663.1 serine hydroxymethyltransferase -
  CHHT_RS03050 (CHHT_0607) - 568701..569243 (+) 543 WP_034962664.1 DUF1882 domain-containing protein -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 39425.20 Da        Isoelectric Point: 6.5030

>NTDB_id=447954 CHHT_RS03025 WP_034962660.1 563826..564893(+) (pilT) [Campylobacter hyointestinalis subsp. hyointestinalis strain CCUG 14169]
MSINIEKLLKTLVYNKGSDLHLVARSEPQIRVSGVLRPLQCGILDGDDVENLCYTVLTDAQKSDLEENKELDFSIELPGI
GRFRGNCYYTMNGSLAAAFRMIPLEIPSLDDLKAPEIFKDIVRREKGLVLVTGPTGSGKSTTLAAMLNEINLTERKHIIT
IEDPIEFVHKNKKSLFSHRNIGTDTKTFASALKYALREDPDIILVGDLRDIEAISIAISAAETGHLVFGTLHTNSAIQTV
NRIVDSFDESEQAQIRNMLSISLHAVISQVLVPLTSGGRIAIHEILINNPAISNLIRENKIHQIYSQLQLNQQNTGMQTQ
TQDMERAFKSGLITKDNALRYSTNKQELIGKIGHL

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=447954 CHHT_RS03025 WP_034962660.1 563826..564893(+) (pilT) [Campylobacter hyointestinalis subsp. hyointestinalis strain CCUG 14169]
ATGAGTATAAATATAGAAAAGCTTCTAAAAACTTTGGTTTATAACAAAGGTAGTGATCTACATCTAGTCGCTAGAAGCGA
ACCTCAGATCCGTGTAAGTGGAGTTTTGAGACCTTTACAGTGCGGAATTTTAGATGGAGATGATGTAGAAAATTTATGTT
ATACGGTGCTTACAGACGCACAAAAAAGCGATCTAGAGGAAAATAAAGAATTAGACTTTTCTATAGAGTTACCTGGCATA
GGTCGTTTTAGGGGAAATTGCTATTATACTATGAACGGTAGTTTAGCCGCGGCTTTTCGTATGATTCCTTTAGAAATCCC
AAGCTTAGATGATCTAAAAGCTCCTGAGATATTTAAGGATATAGTGAGACGAGAAAAAGGGCTTGTTTTGGTCACAGGAC
CTACTGGTAGTGGCAAATCAACTACTTTAGCAGCTATGCTAAATGAGATAAACTTAACTGAGAGAAAGCATATCATTACT
ATAGAAGATCCTATAGAATTTGTTCATAAAAATAAAAAATCTCTCTTTTCTCATAGAAATATAGGAACCGATACAAAGAC
ATTTGCTAGTGCTTTAAAGTACGCTCTTAGGGAAGATCCAGATATTATATTAGTAGGAGATCTGCGCGATATAGAAGCTA
TATCTATCGCGATATCTGCAGCAGAAACAGGGCATTTGGTATTTGGCACTTTGCATACAAACTCAGCCATACAGACTGTA
AATAGGATAGTAGATAGTTTTGATGAGAGCGAGCAAGCTCAGATAAGAAATATGCTTAGTATATCTTTGCATGCTGTCAT
ATCTCAAGTGCTAGTTCCATTAACTAGCGGCGGTAGGATCGCCATACATGAGATACTCATCAATAATCCTGCTATATCAA
ATTTAATAAGAGAGAATAAAATACATCAAATTTATTCTCAGTTGCAGCTAAATCAGCAAAATACCGGTATGCAAACGCAA
ACTCAAGATATGGAAAGAGCATTTAAATCAGGGCTTATAACAAAAGATAATGCTCTTAGATACTCGACAAATAAACAAGA
ATTAATAGGAAAGATAGGGCATTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(17-257)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A855N5J6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

52.738

97.746

0.515
  pilT Neisseria meningitidis 8013

52.45

97.746

0.513
  pilT Vibrio cholerae strain A1552

52.87

93.239

0.493
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.87

93.239

0.493
  pilT Legionella pneumophila strain ERS1305867

50.729

96.62

0.49
  pilT Legionella pneumophila strain Lp02

50.729

96.62

0.49
  pilT Acinetobacter baumannii D1279779

51.19

94.648

0.485
  pilT Acinetobacter baumannii strain A118

51.19

94.648

0.485
  pilT Acinetobacter nosocomialis M2

51.19

94.648

0.485
  pilT Acinetobacter baylyi ADP1

51.36

93.239

0.479
  pilT Pseudomonas aeruginosa PAK

50.595

94.648

0.479
  pilT Pseudomonas stutzeri DSM 10701

50.595

94.648

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.966

98.028

0.431
  pilU Pseudomonas stutzeri DSM 10701

38.701

99.718

0.386
  pilU Acinetobacter baylyi ADP1

37.931

98.028

0.372