Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HPY07_RS01300 Genome accession   NZ_CP053806
Coordinates   278072..279088 (+) Length   338 a.a.
NCBI ID   WP_175241822.1    Uniprot ID   -
Organism   Vibrio cholerae strain SP6G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 273072..284088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY07_RS01285 (HPY07_01285) - 273612..274343 (-) 732 WP_000495226.1 glutathione peroxidase -
  HPY07_RS01290 (HPY07_01290) oxyR 274483..275376 (+) 894 WP_175241820.1 DNA-binding transcriptional regulator OxyR -
  HPY07_RS01295 (HPY07_01295) - 275452..277929 (-) 2478 WP_175241821.1 penicillin-binding protein 1A -
  HPY07_RS01300 (HPY07_01300) pilM 278072..279088 (+) 1017 WP_175241822.1 type IV pilus biogenesis protein PilM Machinery gene
  HPY07_RS01305 (HPY07_01305) pilN 279078..279662 (+) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  HPY07_RS01310 (HPY07_01310) pilO 279655..280245 (+) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  HPY07_RS01315 (HPY07_01315) pilP 280235..280753 (+) 519 WP_032472751.1 pilus assembly protein PilP Machinery gene
  HPY07_RS01320 (HPY07_01320) pilQ 280779..282494 (+) 1716 WP_175241823.1 type IV pilus secretin PilQ Machinery gene
  HPY07_RS01325 (HPY07_01325) aroK 282681..283205 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37684.40 Da        Isoelectric Point: 7.2993

>NTDB_id=447589 HPY07_RS01300 WP_175241822.1 278072..279088(+) (pilM) [Vibrio cholerae strain SP6G]
MGKSLVTGIDIGHNSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSIGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=447589 HPY07_RS01300 WP_175241822.1 278072..279088(+) (pilM) [Vibrio cholerae strain SP6G]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACAACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTCTTTACTTCGGTGCACGGGACTCAAAGTATTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.408

100

0.994
  pilM Vibrio campbellii strain DS40M4

49.112

100

0.491