Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   HPY14_RS01295 Genome accession   NZ_CP053798
Coordinates   278176..278760 (+) Length   194 a.a.
NCBI ID   WP_000902746.1    Uniprot ID   -
Organism   Vibrio cholerae strain SL5Y     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 273176..283760
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY14_RS01280 (HPY14_01280) oxyR 273582..274475 (+) 894 WP_094392824.1 DNA-binding transcriptional regulator OxyR -
  HPY14_RS01285 (HPY14_01285) - 274550..277027 (-) 2478 WP_000668742.1 penicillin-binding protein 1A -
  HPY14_RS01290 (HPY14_01290) pilM 277170..278186 (+) 1017 WP_175233811.1 type IV pilus biogenesis protein PilM Machinery gene
  HPY14_RS01295 (HPY14_01295) pilN 278176..278760 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  HPY14_RS01300 (HPY14_01300) pilO 278753..279343 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  HPY14_RS01305 (HPY14_01305) pilP 279333..279851 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  HPY14_RS01310 (HPY14_01310) pilQ 279877..281592 (+) 1716 WP_162298794.1 type IV pilus secretin PilQ Machinery gene
  HPY14_RS01315 (HPY14_01315) aroK 281779..282303 (+) 525 WP_000818616.1 shikimate kinase AroK -
  HPY14_RS01320 (HPY14_01320) aroB 282331..283416 (+) 1086 WP_000439809.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 22022.45 Da        Isoelectric Point: 9.7294

>NTDB_id=447404 HPY14_RS01295 WP_000902746.1 278176..278760(+) (pilN) [Vibrio cholerae strain SL5Y]
MLHKVNLLPWRDARREAHKRRFLGLVTLGVLLAVLMQFAAGEYLGGQMALQQERIGYLQQHIFSLDQQIAKLKIAEEEHK
ALLTRLTVVEQLQQKRNKTTEFMNQMPNLIPEGVYVDKIKMNGHQIEITGISDSTARLATMLDNLEKSDKLTDVEMHEIV
SGNKRFGKQFQSFKVSFQILTPASNPQEGGAHNG

Nucleotide


Download         Length: 585 bp        

>NTDB_id=447404 HPY14_RS01295 WP_000902746.1 278176..278760(+) (pilN) [Vibrio cholerae strain SL5Y]
ATGTTGCATAAGGTCAACTTACTGCCGTGGCGTGATGCACGGCGTGAAGCACATAAGCGGCGCTTTTTGGGGTTAGTGAC
ACTCGGTGTTTTGCTGGCGGTGTTGATGCAATTTGCCGCAGGCGAATACTTGGGCGGACAAATGGCTTTGCAGCAAGAGC
GGATTGGTTATTTGCAACAACATATCTTCTCGCTGGATCAGCAGATCGCTAAGTTGAAGATCGCTGAAGAAGAACATAAG
GCGTTATTGACCCGTTTGACTGTGGTGGAGCAACTGCAGCAAAAGCGCAATAAAACGACTGAGTTTATGAACCAAATGCC
CAACCTGATCCCTGAAGGCGTCTATGTCGACAAGATCAAGATGAATGGCCATCAGATAGAAATAACCGGGATTAGCGATT
CCACCGCTCGCTTGGCAACCATGCTCGATAACTTGGAGAAGTCGGACAAATTAACCGATGTTGAAATGCATGAAATCGTT
TCAGGGAATAAGCGATTTGGTAAGCAATTTCAGAGCTTTAAAGTCTCCTTCCAAATTTTAACCCCAGCCTCTAACCCGCA
AGAGGGAGGTGCACATAATGGCTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

99.485

100

0.995
  pilN Vibrio campbellii strain DS40M4

60.452

91.237

0.552