Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HPY14_RS01290 Genome accession   NZ_CP053798
Coordinates   277170..278186 (+) Length   338 a.a.
NCBI ID   WP_175233811.1    Uniprot ID   -
Organism   Vibrio cholerae strain SL5Y     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 272170..283186
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY14_RS01275 (HPY14_01275) - 272711..273442 (-) 732 WP_000495226.1 glutathione peroxidase -
  HPY14_RS01280 (HPY14_01280) oxyR 273582..274475 (+) 894 WP_094392824.1 DNA-binding transcriptional regulator OxyR -
  HPY14_RS01285 (HPY14_01285) - 274550..277027 (-) 2478 WP_000668742.1 penicillin-binding protein 1A -
  HPY14_RS01290 (HPY14_01290) pilM 277170..278186 (+) 1017 WP_175233811.1 type IV pilus biogenesis protein PilM Machinery gene
  HPY14_RS01295 (HPY14_01295) pilN 278176..278760 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  HPY14_RS01300 (HPY14_01300) pilO 278753..279343 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  HPY14_RS01305 (HPY14_01305) pilP 279333..279851 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  HPY14_RS01310 (HPY14_01310) pilQ 279877..281592 (+) 1716 WP_162298794.1 type IV pilus secretin PilQ Machinery gene
  HPY14_RS01315 (HPY14_01315) aroK 281779..282303 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37764.49 Da        Isoelectric Point: 7.3328

>NTDB_id=447403 HPY14_RS01290 WP_175233811.1 277170..278186(+) (pilM) [Vibrio cholerae strain SL5Y]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELNLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLSGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=447403 HPY14_RS01290 WP_175233811.1 277170..278186(+) (pilM) [Vibrio cholerae strain SL5Y]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGTGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAACCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTAGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.408

100

0.994
  pilM Vibrio campbellii strain DS40M4

48.225

100

0.482