Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HPY12_RS01300 Genome accession   NZ_CP053794
Coordinates   277047..278063 (+) Length   338 a.a.
NCBI ID   WP_001898302.1    Uniprot ID   -
Organism   Vibrio cholerae strain W10G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 272047..283063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY12_RS01285 (HPY12_01285) - 272587..273318 (-) 732 WP_000495223.1 glutathione peroxidase -
  HPY12_RS01290 (HPY12_01290) oxyR 273458..274351 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  HPY12_RS01295 (HPY12_01295) - 274427..276904 (-) 2478 WP_000668743.1 penicillin-binding protein 1A -
  HPY12_RS01300 (HPY12_01300) pilM 277047..278063 (+) 1017 WP_001898302.1 type IV pilus assembly protein PilM Machinery gene
  HPY12_RS01305 (HPY12_01305) pilN 278053..278637 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  HPY12_RS01310 (HPY12_01310) pilO 278630..279220 (+) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  HPY12_RS01315 (HPY12_01315) pilP 279210..279728 (+) 519 WP_057568694.1 pilus assembly protein PilP Machinery gene
  HPY12_RS01320 (HPY12_01320) pilQ 279754..281469 (+) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  HPY12_RS01325 (HPY12_01325) aroK 281656..282180 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37735.49 Da        Isoelectric Point: 7.3328

>NTDB_id=447307 HPY12_RS01300 WP_001898302.1 277047..278063(+) (pilM) [Vibrio cholerae strain W10G]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLVRSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=447307 HPY12_RS01300 WP_001898302.1 277047..278063(+) (pilM) [Vibrio cholerae strain W10G]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGTCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTACCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCAGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.704

100

0.997
  pilM Vibrio campbellii strain DS40M4

48.521

100

0.485