NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	HPQ66_RS11950	Genome accession	NZ_CP053764
Coordinates	2519170..2519784 (-)	Length	204 a.a.
NCBI ID	WP_013352942.1	Uniprot ID	A0A411A874
Organism	Bacillus velezensis strain B268
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2514170..2524784

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HPQ66_RS11920 (HPQ66_11960)	rpsT	2514470..2514736 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
HPQ66_RS11925 (HPQ66_11965)	holA	2514753..2515794 (-)	1042	Protein_2318	DNA polymerase III subunit delta	-
HPQ66_RS11930 (HPQ66_11970)	-	2515834..2515988 (-)	155	Protein_2319	hypothetical protein	-
HPQ66_RS11935 (HPQ66_11975)	-	2516029..2516163 (+)	135	WP_003152870.1	YqzM family protein	-
HPQ66_RS11940 (HPQ66_11980)	comEC	2516182..2518533 (-)	2352	WP_172614838.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
HPQ66_RS11945 (HPQ66_11985)	-	2518534..2519103 (-)	570	WP_003152868.1	ComE operon protein 2	-
HPQ66_RS11950 (HPQ66_11990)	comEA	2519170..2519784 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
HPQ66_RS11955 (HPQ66_11995)	comER	2519842..2520663 (+)	822	WP_071391594.1	late competence protein ComER	-
HPQ66_RS11960 (HPQ66_12000)	-	2520732..2521469 (-)	738	WP_172614839.1	class I SAM-dependent methyltransferase	-
HPQ66_RS11965 (HPQ66_12005)	rsfS	2521466..2521822 (-)	357	WP_003152864.1	ribosome silencing factor	-
HPQ66_RS11970 (HPQ66_12010)	yqeK	2521840..2522400 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
HPQ66_RS11975 (HPQ66_12015)	-	2522390..2522959 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
HPQ66_RS11980 (HPQ66_12020)	yhbY	2522970..2523260 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
HPQ66_RS11985 (HPQ66_12025)	aroE	2523254..2524096 (-)	843	WP_172614840.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22190.27 Da Isoelectric Point: 5.4841

                                >NTDB_id=446999 HPQ66_RS11950 WP_013352942.1 2519170..2519784(-) (comEA) [Bacillus velezensis strain B268]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=446999 HPQ66_RS11950 WP_013352942.1 2519170..2519784(-) (comEA) [Bacillus velezensis strain B268]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCCTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCCT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG

CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A411A874

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0446999