Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HPY04_RS01375 Genome accession   NZ_CP053744
Coordinates   296153..296743 (+) Length   196 a.a.
NCBI ID   WP_175248680.1    Uniprot ID   -
Organism   Vibrio cholerae strain SA3G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 291153..301743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY04_RS01360 (HPY04_01360) - 291950..294427 (-) 2478 WP_000668736.1 penicillin-binding protein 1A -
  HPY04_RS01365 (HPY04_01365) pilM 294570..295586 (+) 1017 WP_000520446.1 type IV pilus assembly protein PilM Machinery gene
  HPY04_RS01370 (HPY04_01370) pilN 295576..296160 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  HPY04_RS01375 (HPY04_01375) pilO 296153..296743 (+) 591 WP_175248680.1 type 4a pilus biogenesis protein PilO Machinery gene
  HPY04_RS01380 (HPY04_01380) pilP 296733..297251 (+) 519 WP_102787054.1 pilus assembly protein PilP Machinery gene
  HPY04_RS01385 (HPY04_01385) pilQ 297277..298992 (+) 1716 WP_175248681.1 type IV pilus secretin PilQ Machinery gene
  HPY04_RS01390 (HPY04_01390) aroK 299179..299703 (+) 525 WP_000818616.1 shikimate kinase AroK -
  HPY04_RS01395 (HPY04_01395) aroB 299731..300816 (+) 1086 WP_001960111.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22893.27 Da        Isoelectric Point: 4.4169

>NTDB_id=446769 HPY04_RS01375 WP_175248680.1 296153..296743(+) (pilO) [Vibrio cholerae strain SA3G]
MASLQELELDEISEWPLLPQLVVILLIMLLIQGAGYWFYLMPKQDEISLLKQEEETVKATLRIKANKVAVLPQIQAQLDE
LKERYDFLLRQLPVQKELASMLASVNQLGLDSSLTFTRIDWGERESQEFLYRLPLNIELTGSYHDIGDFSQAIAKLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKQEVDDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=446769 HPY04_RS01375 WP_175248680.1 296153..296743(+) (pilO) [Vibrio cholerae strain SA3G]
ATGGCTAGTTTGCAAGAGCTAGAACTGGATGAAATTAGTGAATGGCCGCTGCTGCCTCAGTTGGTGGTGATTCTGCTTAT
CATGCTACTGATCCAAGGTGCTGGTTATTGGTTCTATCTCATGCCCAAACAAGACGAGATTTCGCTGTTAAAGCAGGAAG
AGGAAACCGTCAAGGCCACACTGCGCATCAAAGCCAATAAAGTGGCGGTATTGCCGCAGATCCAAGCCCAGTTGGACGAG
CTCAAAGAGCGTTACGATTTTTTACTGCGCCAGCTACCGGTGCAAAAAGAGTTAGCCAGCATGCTGGCGTCAGTCAACCA
GCTTGGGCTCGATAGTTCGCTTACCTTTACGCGGATTGATTGGGGGGAGCGTGAAAGCCAAGAGTTTCTTTACCGCTTAC
CGCTCAATATCGAACTGACGGGAAGCTATCACGATATTGGCGATTTTTCGCAAGCGATTGCCAAGTTACCGCGCATCATC
AACTTCGACGATGTGGACTGGCAGCGGGTCAGCCAAGAGAGTAGCACGCTGCACTTTCGAGTTAGAGCCTACACCTACCA
ATTTAAGCAGGAGGTGGATGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(50-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

99.49

100

0.995
  pilO Vibrio campbellii strain DS40M4

71.958

96.429

0.694