Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HPY04_RS01365 Genome accession   NZ_CP053744
Coordinates   294570..295586 (+) Length   338 a.a.
NCBI ID   WP_000520446.1    Uniprot ID   -
Organism   Vibrio cholerae strain SA3G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 289570..300586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY04_RS01350 (HPY04_01350) - 290110..290841 (-) 732 WP_000495226.1 glutathione peroxidase -
  HPY04_RS01355 (HPY04_01355) oxyR 290981..291874 (+) 894 WP_175248679.1 DNA-binding transcriptional regulator OxyR -
  HPY04_RS01360 (HPY04_01360) - 291950..294427 (-) 2478 WP_000668736.1 penicillin-binding protein 1A -
  HPY04_RS01365 (HPY04_01365) pilM 294570..295586 (+) 1017 WP_000520446.1 type IV pilus assembly protein PilM Machinery gene
  HPY04_RS01370 (HPY04_01370) pilN 295576..296160 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  HPY04_RS01375 (HPY04_01375) pilO 296153..296743 (+) 591 WP_175248680.1 type 4a pilus biogenesis protein PilO Machinery gene
  HPY04_RS01380 (HPY04_01380) pilP 296733..297251 (+) 519 WP_102787054.1 pilus assembly protein PilP Machinery gene
  HPY04_RS01385 (HPY04_01385) pilQ 297277..298992 (+) 1716 WP_175248681.1 type IV pilus secretin PilQ Machinery gene
  HPY04_RS01390 (HPY04_01390) aroK 299179..299703 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37735.45 Da        Isoelectric Point: 7.1127

>NTDB_id=446767 HPY04_RS01365 WP_000520446.1 294570..295586(+) (pilM) [Vibrio cholerae strain SA3G]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVQVTEKSLVRSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=446767 HPY04_RS01365 WP_000520446.1 294570..295586(+) (pilM) [Vibrio cholerae strain SA3G]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCCAAGTGACGGAAAAAAGCCTCGTCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCA
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
GTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.408

100

0.994
  pilM Vibrio campbellii strain DS40M4

48.225

100

0.482