Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I5M77_RS16775 Genome accession   NZ_CP065369
Coordinates   3513139..3514245 (+) Length   368 a.a.
NCBI ID   WP_005461714.1    Uniprot ID   Q87LJ8
Organism   Vibrio parahaemolyticus strain 20-082A3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3508139..3519245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5M77_RS16740 (I5M77_16740) - 3508209..3508811 (-) 603 WP_025792780.1 XTP/dITP diphosphatase -
  I5M77_RS16745 (I5M77_16745) - 3508929..3509360 (-) 432 WP_025792781.1 DUF4426 domain-containing protein -
  I5M77_RS16750 (I5M77_16750) yggU 3509488..3509778 (-) 291 WP_005482461.1 DUF167 family protein YggU -
  I5M77_RS16755 (I5M77_16755) - 3509778..3510335 (-) 558 WP_005482440.1 YggT family protein -
  I5M77_RS16760 (I5M77_16760) proC 3510388..3511206 (-) 819 WP_025792783.1 pyrroline-5-carboxylate reductase -
  I5M77_RS16765 (I5M77_16765) - 3511326..3512039 (-) 714 WP_021450047.1 YggS family pyridoxal phosphate-dependent enzyme -
  I5M77_RS16770 (I5M77_16770) pilT 3512068..3513108 (+) 1041 WP_005461712.1 type IV pilus twitching motility protein PilT Machinery gene
  I5M77_RS16775 (I5M77_16775) pilU 3513139..3514245 (+) 1107 WP_005461714.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I5M77_RS16780 (I5M77_16780) ruvX 3514307..3514732 (-) 426 WP_005461715.1 Holliday junction resolvase RuvX -
  I5M77_RS16785 (I5M77_16785) - 3514781..3515344 (-) 564 WP_025792786.1 YqgE/AlgH family protein -
  I5M77_RS16790 (I5M77_16790) gshB 3515458..3516408 (-) 951 WP_005425823.1 glutathione synthase -
  I5M77_RS16795 (I5M77_16795) rsmE 3516423..3517154 (-) 732 WP_005461719.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  I5M77_RS16800 (I5M77_16800) endA 3517279..3517974 (-) 696 WP_005493878.1 deoxyribonuclease I -
  I5M77_RS16805 (I5M77_16805) - 3518094..3518591 (-) 498 WP_020835372.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41415.65 Da        Isoelectric Point: 7.0235

>NTDB_id=446482 I5M77_RS16775 WP_005461714.1 3513139..3514245(+) (pilU) [Vibrio parahaemolyticus strain 20-082A3]
MDLNKFLEGMLTLKASDLYITVGAPILFRVDGELRPQGEKLTEHDVAKLLDSAMDPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETIIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVIGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEPFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=446482 I5M77_RS16775 WP_005461714.1 3513139..3514245(+) (pilU) [Vibrio parahaemolyticus strain 20-082A3]
ATGGATCTGAATAAATTTCTTGAAGGTATGTTGACGCTAAAAGCGTCGGATCTTTACATCACGGTTGGTGCGCCAATTCT
ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAGAAACTCACCGAACATGATGTTGCAAAGTTGCTTGATAGCGCAA
TGGATCCGGATCGGCGTCAGGAGTTTCGCAAAAGCCGCGAATCAAACTTTGCTATCGTCAGAGATTGTGGTCGCTTCCGT
GTTAGTGCTTTTTTTCAGCGAGAACTGCCGGGTGCAGTTATTCGCCGTATCGAAACCATCATTCCTACTTTTGAGCAGTT
AAAACTGCCTTTAGTGCTGCAAGACTTAGCGATTGCCAAGCGTGGTTTGGTGCTGGTGGTCGGCGCGACTGGCTCGGGTA
AATCGACCACGATGGCGGCGATGACGGGCTATCGAAACAGCAACAAAACCGGTCACATCTTGACGGTCGAAGATCCGATC
GAATTTGTGCATGAGCATAAGCGTTGTATCGTGACTCAGCGTGAGGTGGGTTTGGATACCGAAAGCTATGAAGTCGCACT
GAAAAACTCGTTGCGCCAAGCGCCAGATATGATTTTGATTGGCGAGATCCGTAGCCGAGAAACGATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACTTGTGTATGGCAACGTTGCACGCCAACAACGCCAACCAAGCGTTAGAGCGTATTCTCCAT
TTGGTGCCTAAAGATCAAAAAGAGCAATTCTTGTTTGATTTGTCGATGAACCTAAAAGGCGTGATTGGTCAGCAGCTGAT
CCGTGATAAGAATGGTCAAGGGCGACATGGTGTGTTTGAGATCTTGCTAAACAGTCCACGAGTGTCGGATTTGATCCGTC
GTGGTGATTTACACGAATTGAAATCAACCATGGCACGATCCAATGAGTTTGGTATGCTGACGTTTGACCAATCGCTCTAC
AAGCTGGTGATGCAAGGTAAGATCAGCGAAGAGGATGCACTGCACAGTGCGGATTCTGCCAACGATCTTCGTTTAATGCT
GAAAACACAGCGTGGTGAGCCGTTCTCTACAGGCAGTCTTGCCAACGTCAAAATCGATATGGATTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(116-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87LJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

83.152

100

0.832
  pilU Pseudomonas stutzeri DSM 10701

58

95.109

0.552
  pilU Acinetobacter baylyi ADP1

55.271

95.38

0.527
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.231

88.315

0.399
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Acinetobacter nosocomialis M2

42.478

92.12

0.391
  pilT Acinetobacter baumannii D1279779

42.478

92.12

0.391
  pilT Acinetobacter baumannii strain A118

42.478

92.12

0.391
  pilT Pseudomonas aeruginosa PAK

42.183

92.12

0.389
  pilT Acinetobacter baylyi ADP1

41.642

92.663

0.386
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Neisseria gonorrhoeae MS11

40.361

90.217

0.364
  pilT Neisseria meningitidis 8013

40.361

90.217

0.364