Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   C270_RS01290 Genome accession   NC_018673
Coordinates   237671..238948 (+) Length   425 a.a.
NCBI ID   WP_014973747.1    Uniprot ID   K0D8H6
Organism   Leuconostoc carnosum JB16     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 232671..243948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C270_RS01275 (C270_01055) - 233622..235757 (+) 2136 WP_014973744.1 RNA degradosome polyphosphate kinase -
  C270_RS01280 (C270_01060) - 235754..236725 (+) 972 WP_014973745.1 Ppx/GppA family phosphatase -
  C270_RS01285 (C270_01065) - 236766..237401 (-) 636 WP_014973746.1 YigZ family protein -
  C270_RS01290 (C270_01070) comFA 237671..238948 (+) 1278 WP_014973747.1 DEAD/DEAH box helicase family protein Machinery gene
  C270_RS09085 comFC/cflB 239206..239616 (+) 411 WP_223822752.1 phosphoribosyltransferase family protein Machinery gene
  C270_RS01300 (C270_01080) raiA 239699..240241 (+) 543 WP_014973749.1 ribosome-associated translation inhibitor RaiA -
  C270_RS01305 (C270_01085) - 240430..241980 (+) 1551 WP_014973750.1 ABC transporter permease -
  C270_RS01310 (C270_01090) - 242062..242652 (+) 591 WP_014973751.1 TetR/AcrR family transcriptional regulator -
  C270_RS08805 (C270_01095) rpmG 242705..242854 (+) 150 WP_014973752.1 50S ribosomal protein L33 -
  C270_RS01315 (C270_01100) secE 242866..243039 (+) 174 WP_014973753.1 preprotein translocase subunit SecE -
  C270_RS01320 (C270_01105) nusG 243157..243786 (+) 630 WP_014973754.1 transcription termination/antitermination protein NusG -

Sequence

Protein


Download         Length: 425 a.a.        Molecular weight: 48475.33 Da        Isoelectric Point: 10.4556

>NTDB_id=44642 C270_RS01290 WP_014973747.1 237671..238948(+) (comFA) [Leuconostoc carnosum JB16]
MEEYYGRQLVQPKIKNVPAYVSVAATFNNHICQRCGQTDIEKLPNYHFYCRACLALGRVSSRDVLLSLPEPNLFDGHDIL
SWSGKLTAKQQEVSNELIATLKEKNEHLVWAVTGAGKTEMLFPVIHKALTQRYRVGIVSPRVDVIIELAPRLQAAFSKTD
MVVLHGEQTRDYRYTPLVLATTHQMLRFKSAFDLLIVDEVDSFPYAGDKMLSYAVQQAKKATGTLIFLTATPTKQLQKRV
RRGSLKTSYLPLRYHQHLLPVIKTTLVGNWRRKIPKQLVKQLTHFQRTEQRFLIFVPKVADLKKVYDQINKHVPHLKGDY
VHAADSRRQEKVQAMRDYQLQYLVTTTILERGVTFPGIDVLILGADDTTFSENALVQIAGRVGRKNDRPTGLVRAYVQHI
SLNVVAAQRQIKQMNHRGKKLGGRK

Nucleotide


Download         Length: 1278 bp        

>NTDB_id=44642 C270_RS01290 WP_014973747.1 237671..238948(+) (comFA) [Leuconostoc carnosum JB16]
ATGGAAGAATATTACGGCAGGCAACTTGTACAACCAAAAATAAAAAATGTGCCAGCTTATGTATCAGTAGCGGCGACGTT
TAATAATCACATTTGTCAACGCTGCGGGCAAACTGATATTGAAAAACTACCAAACTATCATTTTTATTGCCGTGCTTGTT
TAGCTTTAGGACGCGTTAGTTCGCGTGATGTTTTGTTATCTTTGCCAGAGCCGAATCTCTTTGATGGTCACGATATTTTA
AGTTGGTCAGGAAAACTAACTGCGAAACAACAAGAAGTTAGTAATGAACTTATCGCGACATTAAAAGAAAAAAATGAACA
TTTAGTTTGGGCAGTTACGGGTGCAGGAAAGACTGAAATGTTGTTTCCGGTGATACACAAAGCGTTGACGCAACGATATC
GTGTTGGGATTGTTTCACCTAGGGTCGACGTGATTATCGAATTAGCGCCACGATTACAGGCTGCCTTTTCAAAAACTGAT
ATGGTCGTGTTACACGGAGAACAAACACGAGACTATCGCTATACACCTTTGGTTTTAGCAACAACACATCAGATGTTACG
TTTTAAATCTGCTTTTGATTTGTTAATTGTTGATGAAGTTGATAGCTTTCCTTACGCAGGCGATAAAATGTTATCTTATG
CGGTGCAACAAGCTAAAAAGGCTACTGGAACGTTGATTTTTCTAACAGCGACACCTACAAAACAATTACAAAAACGTGTT
CGGAGAGGTTCATTAAAAACATCTTACTTACCGTTGAGGTATCATCAACATTTGTTACCGGTAATAAAAACAACATTAGT
TGGTAATTGGCGACGAAAGATACCGAAGCAGTTGGTCAAACAACTGACTCATTTTCAACGTACAGAACAACGTTTTTTAA
TATTTGTGCCGAAAGTAGCTGATTTAAAAAAAGTGTATGATCAGATTAACAAGCATGTGCCACATTTAAAAGGCGACTAT
GTTCATGCGGCAGATAGTAGACGACAAGAAAAAGTGCAAGCTATGCGAGATTATCAGTTGCAATATCTTGTCACCACAAC
GATTTTGGAACGAGGTGTCACTTTTCCAGGCATTGATGTGTTAATTTTAGGCGCTGATGATACGACTTTTAGTGAAAATG
CTTTAGTTCAAATTGCCGGACGCGTTGGGCGTAAAAATGATCGGCCAACGGGATTGGTACGAGCTTACGTGCAACATATT
AGCTTAAATGTGGTAGCTGCTCAAAGGCAAATTAAACAAATGAATCATCGAGGTAAGAAATTAGGAGGACGAAAATGA

Domains

Predicted by InterProScan.

Type III restriction enzyme, res subunit (ResIII)

(85-233)

Helicase conserved C-terminal domain (Helicase_C)

(277-385)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K0D8H6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

42.627

100

0.435
  comFA Latilactobacillus sakei subsp. sakei 23K

42.823

98.353

0.421
  comFA Lactococcus lactis subsp. cremoris KW2

39.904

97.882

0.391
  comFA/cflA Streptococcus pneumoniae Rx1

41.837

92.235

0.386
  comFA/cflA Streptococcus pneumoniae D39

41.837

92.235

0.386
  comFA/cflA Streptococcus pneumoniae R6

41.837

92.235

0.386
  comFA/cflA Streptococcus mitis SK321

41.837

92.235

0.386
  comFA/cflA Streptococcus pneumoniae TIGR4

41.837

92.235

0.386
  comFA/cflA Streptococcus mitis NCTC 12261

41.071

92.235

0.379

Multiple sequence alignment