Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HPK20_RS13450 Genome accession   NZ_CP053664
Coordinates   2892791..2893381 (-) Length   196 a.a.
NCBI ID   WP_171933976.1    Uniprot ID   -
Organism   Vibrio fluvialis strain A8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2887791..2898381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPK20_RS13430 (HPK20_13450) aroB 2888709..2889794 (-) 1086 WP_020329448.1 3-dehydroquinate synthase -
  HPK20_RS13435 (HPK20_13455) aroK 2889822..2890340 (-) 519 WP_004728569.1 shikimate kinase AroK -
  HPK20_RS13440 (HPK20_13460) pilQ 2890528..2892264 (-) 1737 WP_233921186.1 type IV pilus secretin PilQ Machinery gene
  HPK20_RS13445 (HPK20_13465) pilP 2892289..2892801 (-) 513 WP_020329446.1 pilus assembly protein PilP Machinery gene
  HPK20_RS13450 (HPK20_13470) pilO 2892791..2893381 (-) 591 WP_171933976.1 type 4a pilus biogenesis protein PilO Machinery gene
  HPK20_RS13455 (HPK20_13475) pilN 2893374..2893964 (-) 591 WP_100267596.1 PilN domain-containing protein Machinery gene
  HPK20_RS13460 (HPK20_13480) pilM 2893954..2894958 (-) 1005 WP_171933977.1 type IV pilus assembly protein PilM Machinery gene
  HPK20_RS13465 (HPK20_13485) - 2895101..2897581 (+) 2481 WP_032081685.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22752.13 Da        Isoelectric Point: 4.7023

>NTDB_id=446140 HPK20_RS13450 WP_171933976.1 2892791..2893381(-) (pilO) [Vibrio fluvialis strain A8]
MANLQELELDEIAEWPLLPQLAVLLLLVLALQGAGYWVYMLLKQDALEGLKQQEQTLKSTIRIKASKVATLPKLKTQLDE
LAERYDFLLRQLPVQKELASMLASVNELGLENTLTFTRIDWGERQNQEFLYRLPLNIELTGNYHNIGDFSEAIARLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKPEVSDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=446140 HPK20_RS13450 WP_171933976.1 2892791..2893381(-) (pilO) [Vibrio fluvialis strain A8]
ATGGCTAACTTGCAGGAACTGGAACTGGATGAAATCGCTGAATGGCCTTTGTTGCCTCAGTTGGCCGTGTTGTTACTGCT
GGTTCTGGCGCTGCAAGGTGCGGGCTATTGGGTATATATGTTGCTCAAGCAGGATGCGCTCGAAGGTTTAAAACAGCAGG
AACAAACGCTCAAATCGACGATACGTATCAAAGCCAGCAAAGTGGCCACGTTACCCAAACTCAAAACCCAACTGGATGAA
CTGGCAGAACGGTATGACTTCCTGCTGCGTCAACTGCCGGTGCAAAAAGAGCTTGCCAGTATGCTGGCGTCGGTTAACGA
GTTGGGGCTGGAAAATACGCTGACGTTCACGCGGATTGACTGGGGAGAGAGGCAGAATCAGGAATTCCTCTATCGTCTGC
CACTCAATATCGAACTGACCGGCAATTATCACAACATCGGCGACTTTTCCGAAGCGATTGCGCGCTTGCCACGCATCATC
AACTTTGACGATGTCGATTGGCAGCGCGTCAGTCAGGAGAGCAGTACGCTGCACTTTCGCGTCCGCGCTTACACCTATCA
GTTCAAACCGGAGGTGAGCGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

79.592

100

0.796
  pilO Vibrio campbellii strain DS40M4

67.895

96.939

0.658